1Z3Q

Resolution of the structure of the allergenic and antifungal banana fruit thaumatin-like protein at 1.7A

1Z3Q の概要

• エントリーDOI: 10.2210/pdb1z3q/pdb
• 分子名称: Thaumatin-like Protein, 1,2-ETHANEDIOL (3 entities in total)
• 機能のキーワード: beta sandwich, antifungal protein
• 由来する生物種: Musa acuminata
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21431.71

構造登録者

Leone, P.,Menu-Bouaouiche, L.,Peumans, W.J.,Barre, A.,Payan, F.,Roussel, A.,Van Damme, E.J.M.,Rouge, P. (登録日: 2005-03-14, 公開日: 2006-01-24, 最終更新日: 2024-10-16)

主引用文献

Leone, P.,Menu-Bouaouiche, L.,Peumans, W.J.,Payan, F.,Barre, A.,Roussel, A.,Van Damme, E.J.M.,Rouge, P.
Resolution of the structure of the allergenic and antifungal banana fruit thaumatin-like protein at 1.7-A
Biochimie, 88:45-52, 2006

PubMed Abstract: The structure of a thaumatin-like protein from banana (Musa acuminata) fruit, an allergen with antifungal properties, was solved at 1.7-A-resolution, by X-ray crystallography. Though the banana protein exhibits a very similar overall fold as thaumatin it markedly differs from the sweet-tasting protein by the presence of a surface exposed electronegative cleft. Due to the presence of this electronegative cleft, the banana thaumatin-like protein (Ban-TLP) acquires a strong (local) electronegative character that eventually explains the observed antifungal activity. Our structural analysis also revealed the presence of conserved residues of exposed epitopic determinants that are presumably responsible for the allergenic properties of banana fruit towards susceptible individuals, and provided evidence that the Ban-TLP shares some structurally highly conserved IgE-binding epitopes with thaumatin-like proteins from fruits or pollen from other plants. In addition, some overlap was detected between the predicted IgE-binding epitopes of the Ban-TLP and IgE-binding epitopes previously identified in the mountain cedar Jun a 3 TLP aeroallergen. The presence of these common epitopes offers a molecular basis for the cross-reactivity between aeroallergens and fruit allergens.

PubMed: 16085352
DOI: 10.1016/j.biochi.2005.07.001

実験手法

X-RAY DIFFRACTION (1.7 Å)