1Z14

Structural Determinants of Tissue Tropism and In Vivo Pathogenicity for the Parvovirus Minute Virus of Mice

Summary for 1Z14

• Entry DOI: 10.2210/pdb1z14/pdb
• Descriptor: VP2 (2 entities in total)
• Functional Keywords: minute virus of mice, prototype strain, icosahedral virus, virus
• Biological source: Minute virus of mice
• Total number of polymer chains: 1
• Total formula weight: 61246.07

Authors

Kontou, M.,Govindasamy, L.,Nam, H.J.,Bryant, N.,Llamas-Saiz, A.L.,Foces-Foces, C.,Hernando, E.,Rubio, M.P.,McKenna, R.,Almendral, J.M.,Agbandje-McKenna, M. (deposition date: 2005-03-03, release date: 2005-09-06, Last modification date: 2024-04-03)

Primary citation

Kontou, M.,Govindasamy, L.,Nam, H.J.,Bryant, N.,Llamas-Saiz, A.L.,Foces-Foces, C.,Hernando, E.,Rubio, M.P.,McKenna, R.,Almendral, J.M.,Agbandje-McKenna, M.
Structural determinants of tissue tropism and in vivo pathogenicity for the parvovirus minute virus of mice.
J.Virol., 79:10931-10943, 2005

PubMed Abstract: Two strains of the parvovirus minute virus of mice (MVM), the immunosuppressive (MVMi) and the prototype (MVMp) strains, display disparate in vitro tropism and in vivo pathogenicity. We report the crystal structures of MVMp virus-like particles (MVMp(b)) and native wild-type (wt) empty capsids (MVMp(e)), determined and refined to 3.25 and 3.75 A resolution, respectively, and their comparison to the structure of MVMi, also refined to 3.5 A resolution in this study. A comparison of the MVMp(b) and MVMp(e) capsids showed their structures to be the same, providing structural verification that some heterologously expressed parvovirus capsids are indistinguishable from wt capsids produced in host cells. The structures of MVMi and MVMp capsids were almost identical, but local surface conformational differences clustered from symmetry-related capsid proteins at three specific domains: (i) the icosahedral fivefold axis, (ii) the "shoulder" of the protrusion at the icosahedral threefold axis, and (iii) the area surrounding the depression at the icosahedral twofold axis. The latter two domains contain important determinants of MVM in vitro tropism (residues 317 and 321) and forward mutation residues (residues 399, 460, 553, and 558) conferring fibrotropism on MVMi. Furthermore, these structural differences between the MVM strains colocalize with tropism and pathogenicity determinants mapped for other autonomous parvovirus capsids, highlighting the importance of common parvovirus capsid regions in the control of virus-host interactions.

PubMed: 16103145
DOI: 10.1128/JVI.79.17.10931-10943.2005

Experimental method

X-RAY DIFFRACTION (3.25 Å)