1YY6

The Crystal Structure of the N-terminal domain of HAUSP/USP7 complexed with an EBNA1 peptide

Summary for 1YY6

• Entry DOI: 10.2210/pdb1yy6/pdb
• Descriptor: Ubiquitin carboxyl-terminal hydrolase 7, Epstein-Barr nuclear antigen-1, SODIUM ION, ... (4 entities in total)
• Functional Keywords: traf-domain, peptide binding site, protein peptide complex, hydrolase
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus : Q93009
• Total number of polymer chains: 2
• Total formula weight: 19528.79

Authors

Saridakis, V.,Sheng, Y.,Sarkari, F.,Holowaty, M.,Shire, K.,Nguyen, T.,Zhang, R.,Liao, J.,Lee, W.,Edwards, A.M.,Arrowsmith, C.H.,Frappier, L. (deposition date: 2005-02-23, release date: 2005-04-05, Last modification date: 2024-02-14)

Primary citation

Saridakis, V.,Sheng, Y.,Sarkari, F.,Holowaty, M.N.,Shire, K.,Nguyen, T.,Zhang, R.G.,Liao, J.,Lee, W.,Edwards, A.M.,Arrowsmith, C.H.,Frappier, L.
Structure of the p53 binding domain of HAUSP/USP7 bound to Epstein-Barr nuclear antigen 1 implications for EBV-mediated immortalization.
Mol.Cell, 18:25-36, 2005

PubMed Abstract: USP7/HAUSP is a key regulator of p53 and Mdm2 and is targeted by the Epstein-Barr nuclear antigen 1 (EBNA1) protein of Epstein-Barr virus (EBV). We have determined the crystal structure of the p53 binding domain of USP7 alone and bound to an EBNA1 peptide. This domain is an eight-stranded beta sandwich similar to the TRAF-C domains of TNF-receptor associated factors, although the mode of peptide binding differs significantly from previously observed TRAF-peptide interactions in the sequence (DPGEGPS) and the conformation of the bound peptide. NMR chemical shift analyses of USP7 bound by EBNA1 and p53 indicated that p53 binds the same pocket as EBNA1 but makes less extensive contacts with USP7. Functional studies indicated that EBNA1 binding to USP7 can protect cells from apoptotic challenge by lowering p53 levels. The data provide a structural and conceptual framework for understanding how EBNA1 might contribute to the survival of Epstein-Barr virus-infected cells.

PubMed: 15808506
DOI: 10.1016/j.molcel.2005.02.029

Experimental method

X-RAY DIFFRACTION (1.7 Å)