1YX8

NMR structure of Calsensin, 20 low energy structures.

1YX8 の概要

• エントリーDOI: 10.2210/pdb1yx8/pdb
• 関連するPDBエントリー: 1YX7
• NMR情報: BMRB: 6519
• 分子名称: Calsensin (1 entity in total)
• 機能のキーワード: calsensin, calcium-binding protein, ef-hand, helix-loop-helix, nervous system, metal binding protein
• 由来する生物種: Haemopis marmorata
• 細胞内の位置: Cytoplasm: Q25088
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9115.63

構造登録者

Venkitaramani, D.V.,Fulton, D.B.,Andreotti, A.H.,Johansen, K.M.,Johansen, J. (登録日: 2005-02-19, 公開日: 2005-04-05, 最終更新日: 2024-05-22)

主引用文献

Venkitaramani, D.V.,Fulton, D.B.,Andreotti, A.H.,Johansen, K.M.,Johansen, J.
Solution structure and backbone dynamics of Calsensin, an invertebrate neuronal calcium-binding protein.
Protein Sci., 14:1894-1901, 2005

PubMed Abstract: Calsensin is an EF-hand calcium-binding protein expressed by a subset of peripheral sensory neurons that fasciculate into a single tract in the leech central nervous system. Calsensin is a 9-kD protein with two EF-hand calcium-binding motifs. Using multidimensional NMR spectroscopy we have determined the solution structure and backbone dynamics of calcium-bound Calsensin. Calsensin consists of four helices forming a unicornate-type four-helix bundle. The residues in the third helix undergo slow conformational exchange indicating that the motion of this helix is associated with calciumbinding. The backbone dynamics of the protein as measured by (15)N relaxation rates and heteronuclear NOEs correlate well with the three-dimensional structure. Furthermore, comparison of the structure of Calsensin with other members of the EF-hand calcium-binding protein family provides insight into plausible mechanisms of calcium and target protein binding.

PubMed: 15937283
DOI: 10.1110/ps.051412605

実験手法

SOLUTION NMR