1YSV

Solution structure of the central region of the human GluR-B R/G pre-mRNA

Summary for 1YSV

• Entry DOI: 10.2210/pdb1ysv/pdb
• Descriptor: 27-MER (1 entity in total)
• Functional Keywords: rna, pentaloop, rna editing, adar2, stem-loop
• Total number of polymer chains: 1
• Total formula weight: 8620.15

Authors

Stefl, R.,Allain, F.H. (deposition date: 2005-02-09, release date: 2005-07-19, Last modification date: 2024-05-22)

Primary citation

Stefl, R.,Allain, F.H.
A novel RNA pentaloop fold involved in targeting ADAR2.
Rna, 11:592-597, 2005

PubMed Abstract: Adenosine deaminases that act on RNA (ADARs) catalyze the site-specific conversion of adenosine to inosine in primary mRNA transcripts, thereby affecting coding potential of mature mRNAs. Structural determinants that define the adenosine moieties for specific ADARs-mediated deaminations are currently unknown. We report the solution structure of the central region of the human R/G stem-loop pre-mRNA, a natural ADAR2 substrate encoding the subunit B of the glutamate receptor (R/G site). The structure reveals that the GCU(A/C)A pentaloop that is conserved in mammals and birds adopts a novel fold. The fold is stabilized by a complex interplay of hydrogen bonds and stacking interactions. We propose that this new pentaloop structure is an important determinant of the R/G site recognition by ADAR2.

PubMed: 15840813
DOI: 10.1261/rna.7276805

Experimental method

SOLUTION NMR