1YST

STRUCTURE OF THE PHOTOCHEMICAL REACTION CENTER OF A SPHEROIDENE CONTAINING PURPLE BACTERIUM, RHODOBACTER SPHAEROIDES Y, AT 3 ANGSTROMS RESOLUTION

Summary for 1YST

• Entry DOI: 10.2210/pdb1yst/pdb
• Descriptor: PHOTOSYNTHETIC REACTION CENTER (L SUBUNIT), PHOTOSYNTHETIC REACTION CENTER (M SUBUNIT), PHOTOSYNTHETIC REACTION CENTER (H SUBUNIT), ... (8 entities in total)
• Functional Keywords: photosynthetic reaction center
• Biological source: Rhodobacter sphaeroides; More
• Cellular location: Cellular chromatophore membrane; Multi-pass membrane protein: P02954 P02953; Cellular chromatophore membrane; Single-pass membrane protein: P11846
• Total number of polymer chains: 3
• Total formula weight: 100654.23

Authors

Arnoux, B.,Gaucher, J.F.,Ducruix, A.,Reiss-Husson, F. (deposition date: 1994-12-07, release date: 1995-02-27, Last modification date: 2024-02-14)

Primary citation

Arnoux, B.,Gaucher, J.F.,Ducruix, A.,Reiss-Husson, F.
Structure of the photochemical reaction centre of a spheroidene-containing purple-bacterium, Rhodobacter sphaeroides Y, at 3 A resolution.
Acta Crystallogr.,Sect.D, 51:368-379, 1995

PubMed Abstract: The crystal structure of the photochemical reaction centre from Rhodobacter sphaeroides Y, a carotenoid-containing wild-type purple bacterium, has been determined at 3 A resolution. This membrane complex consists of three subunits (281, 307 and 260 residues, respectively) and ten cofactors. It was crystallized in presence of beta-D-octylglucoside. The crystals are orthorhombic with unit-cell dimensions, a = 143.7, b = 139.8, c = 78.7 A, space group P2(1)2(1)2(1) with four molecules in the unit cell. Refinement of the structure by X-PLOR and manual reconstructions yielded an R value of 22.1% for 19630 reflections between 7 and 3 A. The secondary structure is highly homologous to those determined for Rhodopseudomonas viridis (Protein Data Bank entry 1PRC) and Rhodobacter sphaeroides R26 (Protein Data Bank entry 4RCR) reaction centres. In the latter two structures one Fe(2+) ion located between the two quinones is coordinated by four histidines and one glutamic acid. In the Rhodobacter sphaeroides Y structure, Mn(2+) occupies the same position with identical ligands and geometry. The carotenoid conformation which is a non-planar 15-15'-cis spheroidene molecule in our structure differs from the 13-14-cis 2,4-dihydroneusporene in the Rhodopseudomonas viridis structure.

PubMed: 15299304
DOI: 10.1107/S0907444994013120

Experimental method

X-RAY DIFFRACTION (3 Å)