1YST

STRUCTURE OF THE PHOTOCHEMICAL REACTION CENTER OF A SPHEROIDENE CONTAINING PURPLE BACTERIUM, RHODOBACTER SPHAEROIDES Y, AT 3 ANGSTROMS RESOLUTION

1YST の概要

• エントリーDOI: 10.2210/pdb1yst/pdb
• 分子名称: PHOTOSYNTHETIC REACTION CENTER (L SUBUNIT), PHOTOSYNTHETIC REACTION CENTER (M SUBUNIT), PHOTOSYNTHETIC REACTION CENTER (H SUBUNIT), ... (8 entities in total)
• 機能のキーワード: photosynthetic reaction center
• 由来する生物種: Rhodobacter sphaeroides; 詳細
• 細胞内の位置: Cellular chromatophore membrane; Multi-pass membrane protein: P02954 P02953; Cellular chromatophore membrane; Single-pass membrane protein: P11846
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 100654.23

構造登録者

Arnoux, B.,Gaucher, J.F.,Ducruix, A.,Reiss-Husson, F. (登録日: 1994-12-07, 公開日: 1995-02-27, 最終更新日: 2024-02-14)

主引用文献

Arnoux, B.,Gaucher, J.F.,Ducruix, A.,Reiss-Husson, F.
Structure of the photochemical reaction centre of a spheroidene-containing purple-bacterium, Rhodobacter sphaeroides Y, at 3 A resolution.
Acta Crystallogr.,Sect.D, 51:368-379, 1995

PubMed Abstract: The crystal structure of the photochemical reaction centre from Rhodobacter sphaeroides Y, a carotenoid-containing wild-type purple bacterium, has been determined at 3 A resolution. This membrane complex consists of three subunits (281, 307 and 260 residues, respectively) and ten cofactors. It was crystallized in presence of beta-D-octylglucoside. The crystals are orthorhombic with unit-cell dimensions, a = 143.7, b = 139.8, c = 78.7 A, space group P2(1)2(1)2(1) with four molecules in the unit cell. Refinement of the structure by X-PLOR and manual reconstructions yielded an R value of 22.1% for 19630 reflections between 7 and 3 A. The secondary structure is highly homologous to those determined for Rhodopseudomonas viridis (Protein Data Bank entry 1PRC) and Rhodobacter sphaeroides R26 (Protein Data Bank entry 4RCR) reaction centres. In the latter two structures one Fe(2+) ion located between the two quinones is coordinated by four histidines and one glutamic acid. In the Rhodobacter sphaeroides Y structure, Mn(2+) occupies the same position with identical ligands and geometry. The carotenoid conformation which is a non-planar 15-15'-cis spheroidene molecule in our structure differs from the 13-14-cis 2,4-dihydroneusporene in the Rhodopseudomonas viridis structure.

PubMed: 15299304
DOI: 10.1107/S0907444994013120

実験手法

X-RAY DIFFRACTION (3 Å)