1YRH

Crystal Structure Of Trp Repressor Binding Protein Wrba in complex with FMN

1YRH の概要

• エントリーDOI: 10.2210/pdb1yrh/pdb
• 関連するPDBエントリー: 1YDG
• 分子名称: trp repressor binding protein WrbA, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: alpha-beta twisted open sheet, structural genomics, psi, protein structure initiative, new york sgx research center for structural genomics, nysgxrc, protein binding
• 由来する生物種: Deinococcus radiodurans
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 186856.24

構造登録者

Gorman, J.,Shapiro, L.,Burley, S.K.,New York SGX Research Center for Structural Genomics (NYSGXRC) (登録日: 2005-02-03, 公開日: 2005-02-15, 最終更新日: 2021-02-03)

主引用文献

Gorman, J.,Shapiro, L.
Crystal structures of the tryptophan repressor binding protein WrbA and complexes with flavin mononucleotide.
Protein Sci., 14:3004-3012, 2005

PubMed Abstract: The tryptophan repressor binding protein WrbA binds to the tryptophan repressor protein TrpR. Although the biological role of WrbA remains unclear, it has been proposed to function in enhancing the stability of TrpR-DNA complexes. Sequence database analysis has identified WrbA as a founding member of a flavodoxin-like family of proteins. Here we present crystal structures of WrbA from Deinococcus radiodurans and Pseudomonas aeruginosa and their complexes with flavin mononucleotide. The protomer structure is similar to that of previously determined long-chain flavodoxins; however, each contains a conserved inserted region unique to the WrbA family. Interestingly, each WrbA protein forms a homotetramer with 222 symmetry, unique among flavodoxin-like proteins, in which each protomer binds one flavin mononucleotide cofactor molecule.

PubMed: 16322580
DOI: 10.1110/ps.051680805

実験手法

X-RAY DIFFRACTION (3.11 Å)