1YOW

human Steroidogenic Factor 1 LBD with bound Co-factor Peptide

1YOW の概要

• エントリーDOI: 10.2210/pdb1yow/pdb
• 分子名称: Steroidogenic factor 1, TIF2 peptide, PHOSPHATIDYL ETHANOL, ... (4 entities in total)
• 機能のキーワード: steroidogenic factor 1, sf1, phospholipid, phosphatidyl ethanolamine, phosphatidyl glycerol, transcription
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : Q13285
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29500.58

構造登録者

Krylova, I.N.,Sablin, E.P.,Xu, R.X.,Waitt, G.M.,Juzumiene, D.,Williams, J.D.,Ingraham, H.A.,Willson, T.M.,Williams, S.P.,Montana, V.,Madauss, K.P.,Moore, J.,Bynum, J.M.,Lebedeva, L.,MacKay, J.A.,Suzawa, M.,Guy, R.K.,Thornton, J.W. (登録日: 2005-01-28, 公開日: 2005-05-10, 最終更新日: 2024-02-14)

主引用文献

Krylova, I.N.,Sablin, E.P.,Xu, R.X.,Waitt, G.M.,MacKay, J.A.,Juzumiene, D.,Bynum, J.M.,Madauss, K.P.,Montana, V.,Lebedeva, L.,Suzawa, M.,Williams, J.D.,Williams, S.P.,Guy, R.K.,Thornton, J.W.,Fletterick, R.J.,Willson, T.M.,Ingraham, H.A.
Structural analyses reveal phosphatidyl inositols as ligands for the NR5 orphan receptors SF-1 and LRH-1
Cell(Cambridge,Mass.), 120:343-355, 2005

PubMed Abstract: Vertebrate members of the nuclear receptor NR5A subfamily, which includes steroidogenic factor 1 (SF-1) and liver receptor homolog 1 (LRH-1), regulate crucial aspects of development, endocrine homeostasis, and metabolism. Mouse LRH-1 is believed to be a ligand-independent transcription factor with a large and empty hydrophobic pocket. Here we present structural and biochemical data for three other NR5A members-mouse and human SF-1 and human LRH-1-which reveal that these receptors bind phosphatidyl inositol second messengers and that ligand binding is required for maximal activity. Evolutionary analysis of structure-function relationships across the SF-1/LRH-1 subfamily indicates that ligand binding is the ancestral state of NR5A receptors and was uniquely diminished or altered in the rodent LRH-1 lineage. We propose that phospholipids regulate gene expression by directly binding to NR5A nuclear receptors.

PubMed: 15707893
DOI: 10.1016/j.cell.2005.01.024

実験手法

X-RAY DIFFRACTION (3 Å)