1YK5

Pyrococcus abyssi rubredoxin

Summary for 1YK5

• Entry DOI: 10.2210/pdb1yk5/pdb
• Related: 1YK4
• Descriptor: Rubredoxin, FE (III) ION (3 entities in total)
• Functional Keywords: electron transport
• Biological source: Pyrococcus abyssi
• Total number of polymer chains: 4
• Total formula weight: 24410.29

Authors

Bonisch, H.,Schmidt, C.L.,Bianco, P.,Ladenstein, R. (deposition date: 2005-01-17, release date: 2006-01-17, Last modification date: 2023-10-25)

Primary citation

Bonisch, H.,Schmidt, C.L.,Bianco, P.,Ladenstein, R.
Ultrahigh-resolution study on Pyrococcus abyssi rubredoxin. I. 0.69 A X-ray structure of mutant W4L/R5S.
Acta Crystallogr.,Sect.D, 61:990-1004, 2005

PubMed Abstract: The crystal structure of Pyrococcus abyssi rubredoxin mutant W4L/R5S was solved by direct methods. The model of the air-oxidized protein was refined by partially restrained full-matrix least-squares refinement against intensity data to 0.69 A resolution. This first ultrahigh-resolution structure of a rubredoxin provides very detailed and precise information about the Fe(SCys)(4) centre and its environment, the peptide-backbone stereochemistry, H atoms and hydrogen bonds, static and dynamic disorder, the solvent structure and the electron-density distribution. P. abyssi rubredoxin W4L/R5S is the first of a series of mutants studied by atomic and ultrahigh-resolution X-ray crystallography which are expected to contribute to the understanding of structure-function relationships in iron-sulfur proteins.

PubMed: 15983423
DOI: 10.1107/S090744490501293X

Experimental method

X-RAY DIFFRACTION (1.79 Å)