1YJI

RDC-refined Solution NMR structure of reduced putidaredoxin

1YJI の概要

• エントリーDOI: 10.2210/pdb1yji/pdb
• 関連するPDBエントリー: 1PDX 1YJJ
• 分子名称: Putidaredoxin, FE2/S2 (INORGANIC) CLUSTER (2 entities in total)
• 機能のキーワード: ferredoxin, [2fe-2s], redox, iron-sulfur, electron transfer, cytochrome p450cam, electron transport
• 由来する生物種: Pseudomonas putida
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11604.73

構造登録者

Jain, N.U.,Tjioe, E.,Savidor, A.,Boulie, J. (登録日: 2005-01-14, 公開日: 2005-06-28, 最終更新日: 2024-05-22)

主引用文献

Jain, N.U.,Tjioe, E.,Savidor, A.,Boulie, J.
Redox-dependent structural differences in putidaredoxin derived from homologous structure refinement via residual dipolar couplings.
Biochemistry, 44:9067-9078, 2005

PubMed Abstract: Structural differences in the [2Fe-2S] ferredoxin, putidaredoxin (Pdx), from the camphor hydroxylation pathway of Pseudomonas putida have been investigated as a function of oxidation state of the iron cluster. Pdx is involved in biological electron transfer to cytochrome P450(cam) (CYP101). Redox-dependent differences have been observed previously for Pdx in terms of binding affinities to CYP101, NMR spectral differences, and dynamic properties. To further characterize these differences, structure refinement of both oxidized and reduced Pdx has been carried out using a hybrid approach utilizing paramagnetic distance restraints and NMR orientational restraints in the form of backbone (15)N residual dipolar couplings. Use of these new restraints has improved the structure of oxidized Pdx considerably over the earlier solution NMR structure without RDC restraints, with the new structure now much closer in overall fold to the recently published X-ray crystal structures. We now observe better defined relative orientations of the major secondary structure elements as also of the conformation of the metal binding loop region. Extension of this approach to structure calculation of reduced Pdx has identified structural differences that are primarily localized for residues in the C-terminal interaction domain consisting of the functionally important residue Trp 106 and regions near the metal binding loop in Pdx. These redox-dependent structural differences in Pdx correlate to dynamic changes observed before and may be linked to differences in binding and electron transfer properties between oxidized and reduced Pdx.

PubMed: 15966730
DOI: 10.1021/bi050152c

実験手法

SOLUTION NMR