1YHP

Solution Structure of Ca2+-free DdCAD-1

1YHP の概要

• エントリーDOI: 10.2210/pdb1yhp/pdb
• 分子名称: Calcium-dependent cell adhesion molecule-1 (1 entity in total)
• 機能のキーワード: ddcad-1, cell adhesion, calcium
• 由来する生物種: Dictyostelium discoideum
• 細胞内の位置: Cell membrane: P54657
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23818.54

構造登録者

Lin, Z.,Huang, H.B.,Siu, C.H.,Yang, D.W. (登録日: 2005-01-10, 公開日: 2006-01-31, 最終更新日: 2024-05-29)

主引用文献

Lin, Z.,Sriskanthadevan, S.,Huang, H.B.,Siu, C.H.,Yang, D.W.
Solution structures of the adhesion molecule DdCAD-1 reveal new insights into Ca(2+)-dependent cell-cell adhesion
Nat.Struct.Mol.Biol., 13:1016-1022, 2006

PubMed Abstract: DdCAD-1 is a novel Ca(2+)-dependent cell adhesion molecule that lacks a hydrophobic signal peptide and a transmembrane domain. DdCAD-1 is expressed by the social amoeba Dictyostelium discoideum at the onset of development. It is synthesized as a soluble protein and then transported to the plasma membrane by contractile vacuoles. Here we describe the novel features of the solution structures of Ca(2+)-free and Ca(2+)-bound monomeric DdCAD-1. DdCAD-1 contains two beta-sandwich domains, belonging to the betagamma-crystallin and immunoglobulin fold classes, respectively. Whereas the N-terminal domain has a major role in homophilic binding, the C-terminal domain tethers the protein to the cell membrane. From structural and mutational analyses, we propose a model for the Ca(2+)-bound DdCAD-1 dimer as a basis for understanding DdCAD-1-mediated cell-cell adhesion at the molecular level. Our results provide new insights into Ca(2+)-dependent mechanisms for cell-cell adhesion.

PubMed: 17057715
DOI: 10.1038/nsmb1162

実験手法

SOLUTION NMR