1YFE

Crystal structure of apo fumarase C from Escherichia coli

1YFE の概要

• エントリーDOI: 10.2210/pdb1yfe/pdb
• 関連するPDBエントリー: 1FUO 1FUP 1FUQ
• 分子名称: Fumarate hydratase class II (2 entities in total)
• 機能のキーワード: fumarase, kreb's cycle, apo, allosteric, lyase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm : P05042
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 50548.77

構造登録者

Weaver, T. (登録日: 2004-12-31, 公開日: 2005-10-18, 最終更新日: 2023-08-23)

主引用文献

Weaver, T.
Structure of free fumarase C from Escherichia coli.
Acta Crystallogr.,Sect.D, 61:1395-1401, 2005

PubMed Abstract: Previous crystal structures of fumarase C from Escherichia coli have noted two occupied dicarboxylate-binding sites termed the active site and the B site. Here, the first known fumarase C structure is reported in which both sites are unoccupied by bound ligand. This so-called ;free' crystal form shows conservation of the active-site water in a similar orientation to that reported in other fumarase C crystal structures. More importantly, a shift of His129 has been observed at the B site. This new crystallographic information suggests the use of water as a permanent member of the active site and the use of an imidazole-imidazolium conversion to control access at the allosteric B site.

PubMed: 16204892
DOI: 10.1107/S0907444905024194

実験手法

X-RAY DIFFRACTION (2.19 Å)