1YEH

STRUCTURE OF IGG2A FAB FRAGMENT

1YEH の概要

• エントリーDOI: 10.2210/pdb1yeh/pdb
• 分子名称: FAB FRAGMENT, ZINC ION, ... (4 entities in total)
• 機能のキーワード: catalytic antibody, immunoglobulin c region
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48652.90

構造登録者

Gigant, B.,Knossow, M. (登録日: 1997-05-29, 公開日: 1997-12-03, 最終更新日: 2024-10-30)

主引用文献

Gigant, B.,Charbonnier, J.B.,Eshhar, Z.,Green, B.S.,Knossow, M.
X-ray structures of a hydrolytic antibody and of complexes elucidate catalytic pathway from substrate binding and transition state stabilization through water attack and product release.
Proc.Natl.Acad.Sci.USA, 94:7857-7861, 1997

PubMed Abstract: The x-ray structures of the unliganded esterase-like catalytic antibody D2.3 and its complexes with a substrate analogue and with one of the reaction products are analyzed. Together with the structure of the phosphonate transition state analogue hapten complex, these crystal structures provide a complete description of the reaction pathway. At alkaline pH, D2.3 acts by preferential stabilization of the negatively charged oxyanion intermediate of the reaction that results from hydroxide attack on the substrate. A tyrosine residue plays a crucial role in catalysis: it activates the ester substrate and, together with an asparagine, it stabilizes the oxyanion intermediate. A canal allows facile diffusion of water molecules to the reaction center that is deeply buried in the structure. Residues bordering this canal provide targets for mutagenesis to introduce a general base in the vicinity of the reaction center.

PubMed: 9223277
DOI: 10.1073/pnas.94.15.7857

実験手法

X-RAY DIFFRACTION (2.55 Å)