1YBL

NMR structure of the i-motif tetramer of d(AACCCC)

1YBL の概要

• エントリーDOI: 10.2210/pdb1ybl/pdb
• 関連するPDBエントリー: 1YBN 1YBR 294D
• 分子名称: 5'-D(*AP*AP*CP*CP*CP*C)-3' (1 entity in total)
• 機能のキーワード: dna; i-motif; hemiprotonated cytidine; tetrahymena; telomer, dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 6952.73

構造登録者

Esmaili, N.,Leroy, J.-L. (登録日: 2004-12-21, 公開日: 2005-01-11, 最終更新日: 2024-05-01)

主引用文献

Esmaili, N.,Leroy, J.-L.
i-motif solution structure and dynamics of the d(AACCCC) and d(CCCCAA) tetrahymena telomeric repeats
Nucleic Acids Res., 33:213-224, 2005

PubMed Abstract: Using NMR methods, we have resolved the i-motif structures formed by d(AACCCC) and by d(CCCCAA), two versions of the DNA sequence repeated in the telomeric regions of the C-rich strand of tetrahymena chromosomes. Both oligonucleotides form fully symmetrical i-motif tetramers built by intercalation of two hemiprotonated duplexes containing four C*C+ pairs. The structures are extremely stable. In the tetramer of d(AACCCC), the outermost C*C+ pairs are formed by the cytidines of the 5' ends of the cytidine tracts. A2 forms an A2*A2 (H6trans-N7) pair stacked to C3*C3+ and cross-strand stacked to A1. At 0 degrees C, the lifetimes of the hemiprotonated pairs range from 1 ms for the outermost pair to approximately 1 h for the innermost pairs. The tetramer of d(CCCCAA) adopts two distinct intercalation topologies in slow conformational exchange. One, whose outermost C*C+ pairs are built by the cytidines of the 5' end and the other by those of the 3' end. In both topologies, the adenosine bases are fairly well stacked to the adjacent C*C+ pairs. They are not paired but form symmetrical pseudo-pairs with their H6cis amino proton and N1 nitrogen pointing towards each other.

PubMed: 15647504
DOI: 10.1093/nar/gki160

実験手法

SOLUTION NMR