1Y7N

Solution structure of the second PDZ domain of the human neuronal adaptor X11alpha

1Y7N の概要

• エントリーDOI: 10.2210/pdb1y7n/pdb
• 分子名称: Amyloid beta A4 precursor protein-binding family A member 1 (1 entity in total)
• 機能のキーワード: copper chaperone for superoxide dismutase, neuronal adaptor, protein transport
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9933.56

構造登録者

Duquesne, A.E.,de Ruijter, M.,Brouwer, J.,Drijfhout, J.W.,Nabuurs, S.B.,Spronk, C.A.E.M.,Vuister, G.W.,Ubbink, M.,Canters, G.W. (登録日: 2004-12-09, 公開日: 2005-11-22, 最終更新日: 2024-05-29)

主引用文献

Duquesne, A.E.,de Ruijter, M.,Brouwer, J.,Drijfhout, J.W.,Nabuurs, S.B.,Spronk, C.A.E.M.,Vuister, G.W.,Ubbink, M.,Canters, G.W.
Solution structure of the second PDZ domain of the neuronal adaptor X11alpha and its interaction with the C-terminal peptide of the human copper chaperone for superoxide dismutase
J.Biomol.Nmr, 32:209-218, 2005

PubMed Abstract: Protection against reactive oxygen species is provided by the copper containing enzyme superoxide dismutase 1 (SOD1). The copper chaperone CCS is responsible for copper insertion into apo-SOD1. This role is impaired by an interaction between the second PDZ domain (PDZ2alpha) of the neuronal adaptor protein X11alpha and the third domain of CCS (McLoughlin et al. (2001) J. Biol. Chem., 276, 9303-9307). The solution structure of the PDZ2alpha domain has been determined and the interaction with peptides derived from CCS has been explored. PDZ2alpha binds to the last four amino acids of the CCS protein (PAHL) with a dissociation constant of 91 +/- 2 microM. Peptide variants have been used to map the interaction areas on PDZ2alpha for each amino acid, showing an important role for the C-terminal leucine, in line with canonical PDZ-peptide interactions.

PubMed: 16132821
DOI: 10.1007/s10858-005-7333-1

実験手法

SOLUTION NMR