1Y5L

The crystal structure of the NarGHI mutant NarI-H66Y

Summary for 1Y5L

• Entry DOI: 10.2210/pdb1y5l/pdb
• Related: 1Q16 1Y4Z 1Y5I 1Y5N
• Descriptor: Respiratory nitrate reductase 1 alpha chain, Respiratory nitrate reductase 1 beta chain, Respiratory nitrate reductase 1 gamma chain, ... (10 entities in total)
• Functional Keywords: nitrate reduction, membrane protein, electron transfer, q-site, oxidoreductase
• Biological source: Escherichia coli; More
• Total number of polymer chains: 3
• Total formula weight: 228845.37

Authors

Bertero, M.G.,Rothery, R.A.,Boroumand, N.,Palak, M.,Blasco, F.,Ginet, N.,Weiner, J.H.,Strynadka, N.C.J. (deposition date: 2004-12-02, release date: 2005-03-08, Last modification date: 2024-10-30)

Primary citation

Bertero, M.G.,Rothery, R.A.,Boroumand, N.,Palak, M.,Blasco, F.,Ginet, N.,Weiner, J.H.,Strynadka, N.C.J.
Structural and Biochemical Characterization of a Quinol Binding Site of Escherichia coli Nitrate Reductase A
J.Biol.Chem., 280:14836-14843, 2005

PubMed Abstract: The crystal structure of Escherichia coli nitrate reductase A (NarGHI) in complex with pentachlorophenol has been determined to 2.0 A of resolution. We have shown that pentachlorophenol is a potent inhibitor of quinol:nitrate oxidoreductase activity and that it also perturbs the EPR spectrum of one of the hemes located in the membrane anchoring subunit (NarI). This new structural information together with site-directed mutagenesis data, biochemical analyses, and molecular modeling provide the first molecular characterization of a quinol binding and oxidation site (Q-site) in NarGHI. A possible proton conduction pathway linked to electron transfer reactions has also been defined, providing fundamental atomic details of ubiquinol oxidation by NarGHI at the bacterial membrane.

PubMed: 15615728
DOI: 10.1074/jbc.M410457200

Experimental method

X-RAY DIFFRACTION (2.5 Å)