1Y4Z

The crystal structure of Nitrate Reductase A, NarGHI, in complex with the Q-site inhibitor pentachlorophenol

1Y4Z の概要

• エントリーDOI: 10.2210/pdb1y4z/pdb
• 関連するPDBエントリー: 1Q16 1SIW 1Y5I 1Y5L 1Y5N
• 分子名称: Respiratory nitrate reductase 1 alpha chain, PENTACHLOROPHENOL, Respiratory nitrate reductase 1 beta chain, ... (11 entities in total)
• 機能のキーワード: nitrate reduction, electron transfer, membrane protein, q-site, oxidoreductase
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cell membrane; Peripheral membrane protein: P09152 P11349; Cell inner membrane; Multi-pass membrane protein: P11350
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 229365.72

構造登録者

Bertero, M.G.,Rothery, R.A.,Boroumand, N.,Palak, M.,Blasco, F.,Ginet, N.,Weiner, J.H.,Strynadka, N.C.J. (登録日: 2004-12-01, 公開日: 2005-03-08, 最終更新日: 2024-10-30)

主引用文献

Bertero, M.G.,Rothery, R.A.,Boroumand, N.,Palak, M.,Blasco, F.,Ginet, N.,Weiner, J.H.,Strynadka, N.C.J.
Structural and Biochemical Characterization of a Quinol Binding Site of Escherichia coli Nitrate Reductase A
J.Biol.Chem., 280:14836-14843, 2005

PubMed Abstract: The crystal structure of Escherichia coli nitrate reductase A (NarGHI) in complex with pentachlorophenol has been determined to 2.0 A of resolution. We have shown that pentachlorophenol is a potent inhibitor of quinol:nitrate oxidoreductase activity and that it also perturbs the EPR spectrum of one of the hemes located in the membrane anchoring subunit (NarI). This new structural information together with site-directed mutagenesis data, biochemical analyses, and molecular modeling provide the first molecular characterization of a quinol binding and oxidation site (Q-site) in NarGHI. A possible proton conduction pathway linked to electron transfer reactions has also been defined, providing fundamental atomic details of ubiquinol oxidation by NarGHI at the bacterial membrane.

PubMed: 15615728
DOI: 10.1074/jbc.M410457200

実験手法

X-RAY DIFFRACTION (2 Å)