1Y3I

Crystal Structure of Mycobacterium tuberculosis NAD kinase-NAD complex

1Y3I の概要

• エントリーDOI: 10.2210/pdb1y3i/pdb
• 関連するPDBエントリー: 1Y3H
• 分子名称: Inorganic polyphosphate/ATP-NAD kinase, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: nad kinase, polyphosphate, nad, mycobacterium tuberculosis, transferase
• 由来する生物種: Mycobacterium tuberculosis
• 細胞内の位置: Cytoplasm: P0A5S6
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 67678.60

構造登録者

Mori, S.,Yamasaki, M.,Maruyama, Y.,Momma, K.,Kawai, S.,Hashimoto, W.,Mikami, B.,Murata, K. (登録日: 2004-11-25, 公開日: 2005-01-18, 最終更新日: 2024-03-13)

主引用文献

Mori, S.,Yamasaki, M.,Maruyama, Y.,Momma, K.,Kawai, S.,Hashimoto, W.,Mikami, B.,Murata, K.
NAD-binding mode and the significance of intersubunit contact revealed by the crystal structure of Mycobacterium tuberculosis NAD kinase-NAD complex
Biochem.Biophys.Res.Commun., 327:500-508, 2005

PubMed Abstract: NAD kinase is a key enzyme in NADP biosynthesis. We solved the crystal structure of polyphosphate/ATP-NAD kinase from Mycobacterium tuberculosis (Ppnk) complexed with NAD (Ppnk-NAD) at 2.6A resolution using apo-Ppnk structure solved in this work, and revealed the details of the structure and NAD-binding site. Superimposition of tertiary structures of apo-Ppnk and Ppnk-NAD demonstrated a substantial conformational difference in a loop (Ppnk-flexible loop). As a quaternary structure, these Ppnk structures exhibited tetramer as in solution condition. Notably, the Ppnk-flexible loop was involved in the intersubunit contact and probably related to the NAD-binding of the other subunit. Furthermore, the two residues (Asp189, His226) substantially contributed to creating NAD-binding site on the other subunit. The two residues and the residues involved in NAD-binding were conserved. However, residues corresponding to the Ppnk-flexible loop were not conserved, making us to speculate that the Ppnk-flexible loop may be Ppnk-specific.

PubMed: 15629142
DOI: 10.1016/j.bbrc.2004.11.163

実験手法

X-RAY DIFFRACTION (2.6 Å)