1Y2Y

Structural Characterization of Nop10p using Nuclear Magnetic Resonance Spectroscopy

1Y2Y の概要

• エントリーDOI: 10.2210/pdb1y2y/pdb
• 分子名称: Ribosome biogenesis protein Nop10 (1 entity in total)
• 機能のキーワード: box h/aca snorna, snornp, pseudouridine, biosynthetic protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Nucleus, nucleolus: Q6Q547
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6649.74

構造登録者

Khanna, M.,Wu, H.,Johansson, C.,Caizergues-Ferrer, M.,Feigon, J. (登録日: 2004-11-23, 公開日: 2005-12-06, 最終更新日: 2024-05-22)

主引用文献

Khanna, M.,Wu, H.,Johansson, C.,Caizergues-Ferrer, M.,Feigon, J.
Structural study of the H/ACA snoRNP components Nop10p and the 3' hairpin of U65 snoRNA
RNA, 12:40-52, 2006

PubMed Abstract: The H/ACA small nucleolar ribonucleoprotein (snoRNP) complexes guide the modification of uridine to pseudouridine at conserved sites in rRNA. The H/ACA snoRNPs each comprise a target-site-specific snoRNA and four core proteins, Nop10p, Nhp2p, Gar1p, and the pseudouridine synthase, Cbf5p, in yeast. The secondary structure of the H/ACA snoRNAs includes two hairpins that each contain a large internal loop (the pseudouridylation pocket), one or both of which are partially complementary to the target RNA(s). We have determined the solution structure of an RNA hairpin derived from the human U65 box H/ACA snoRNA including the pseudouridylation pocket and adjacent stems, providing the first three-dimensional structural information on these H/ACA snoRNAs. We have also determined the structure of Nop10p and investigated its interaction with RNA using NMR spectroscopy. Nop10p contains a structurally well-defined N-terminal region composed of a beta-hairpin, and the rest of the protein lacks a globular structure. Chemical shift mapping of the interaction of RNA constructs of U65 box H/ACA 3' hairpin with Nop10p shows that the beta-hairpin binds weakly but specifically to RNA. The unstructured region of Nop10p likely interacts with Cbf5p.

PubMed: 16373493
DOI: 10.1261/rna.2221606

実験手法

SOLUTION NMR