1Y26

A-riboswitch-adenine complex

1Y26 の概要

• エントリーDOI: 10.2210/pdb1y26/pdb
• 関連するPDBエントリー: 1Y27
• 分子名称: Vibrio vulnificus A-riboswitch, MAGNESIUM ION, ADENINE, ... (4 entities in total)
• 機能のキーワード: a-riboswitch adenine recognition, rna
• 由来する生物種: Vibrio vulnificus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22945.02

構造登録者

Serganov, A.,Yuan, Y.R.,Patel, D.J. (登録日: 2004-11-20, 公開日: 2004-12-28, 最終更新日: 2024-02-14)

主引用文献

Serganov, A.,Yuan, Y.R.,Pikovskaya, O.,Polonskaia, A.,Malinina, L.,Phan, A.T.,Hobartner, C.,Micura, R.,Breaker, R.R.,Patel, D.J.
Structural Basis for Discriminative Regulation of Gene Expression by Adenine- and Guanine-Sensing mRNAs
Chem.Biol., 11:1729-1741, 2004

PubMed Abstract: Metabolite-sensing mRNAs, or "riboswitches," specifically interact with small ligands and direct expression of the genes involved in their metabolism. Riboswitches contain sensing "aptamer" modules, capable of ligand-induced structural changes, and downstream regions, harboring expression-controlling elements. We report the crystal structures of the add A-riboswitch and xpt G-riboswitch aptamer modules that distinguish between bound adenine and guanine with exquisite specificity and modulate expression of two different sets of genes. The riboswitches form tuning fork-like architectures, in which the prongs are held in parallel through hairpin loop interactions, and the internal bubble zippers up to form the purine binding pocket. The bound purines are held by hydrogen bonding interactions involving conserved nucleotides along their entire periphery. Recognition specificity is associated with Watson-Crick pairing of the encapsulated adenine and guanine ligands with uridine and cytosine, respectively.

PubMed: 15610857
DOI: 10.1016/j.chembiol.2004.11.018

実験手法

X-RAY DIFFRACTION (2.1 Å)