1Y14

Crystal structure of yeast subcomplex of Rpb4 and Rpb7

1Y14 の概要

• エントリーDOI: 10.2210/pdb1y14/pdb
• 分子名称: DNA-directed RNA polymerase II 32 kDa polypeptide, DNA-directed RNA polymerase II 19 kDa polypeptide (3 entities in total)
• 機能のキーワード: transferase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Nucleus: P20433 P34087
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 81035.52

構造登録者

Armache, K.-J.,Mitterweger, S.,Meinhart, A.,Cramer, P. (登録日: 2004-11-17, 公開日: 2004-12-14, 最終更新日: 2024-03-13)

主引用文献

Armache, K.-J.,Mitterweger, S.,Meinhart, A.,Cramer, P.
Structures of Complete RNA Polymerase II and Its Subcomplex, Rpb4/7
J.Biol.Chem., 280:7131-7134, 2005

PubMed Abstract: We determined the x-ray structure of the RNA polymerase (Pol) II subcomplex Rpb4/7 at 2.3 A resolution, combined it with a previous structure of the 10-subunit polymerase core, and refined an atomic model of the complete 12-subunit Pol II at 3.8-A resolution. Comparison of the complete Pol II structure with structures of the Pol II core and free Rpb4/7 shows that the core-Rpb4/7 interaction goes along with formation of an alpha-helix in the linker region of the largest Pol II subunit and with folding of the conserved Rpb7 tip loop. Details of the core-Rpb4/7 interface explain facilitated Rpb4/7 dissociation in a temperature-sensitive Pol II mutant and specific assembly of Pol I with its Rpb4/7 counterpart, A43/14. The refined atomic model of Pol II serves as the new reference structure for analysis of the transcription mechanism and enables structure solution of complexes of the complete enzyme with additional factors and nucleic acids by molecular replacement.

PubMed: 15591044
DOI: 10.1074/jbc.M413038200

実験手法

X-RAY DIFFRACTION (2.3 Å)