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1XV7

Solution structure of antimicrobial and endotoxin-neutralizing peptide Lf11 in DPC micelles

Summary for 1XV7
Entry DOI10.2210/pdb1xv7/pdb
Related1XV4
Descriptorlactoferrin-based synthetic peptide FQWQRNIRKVR (1 entity in total)
Functional Keywordsloop, hydrophobic core, metal transport
Biological sourceHomo sapiens (human)
Total number of polymer chains1
Total formula weight1532.82
Authors
Japelj, B.,Pristovsek, P.,Majerle, A.,Jerala, R. (deposition date: 2004-10-27, release date: 2005-03-22, Last modification date: 2024-10-23)
Primary citationJapelj, B.,Pristovsek, P.,Majerle, A.,Jerala, R.
Structural origin of endotoxin neutralization and antimicrobial activity of a lactoferrin-based peptide
J.Biol.Chem., 280:16955-16961, 2005
Cited by
PubMed Abstract: Treatment of Gram-negative bacterial infections with antimicrobial agents can cause release of the endotoxin lipopolysaccharide (LPS), the potent initiator of sepsis, which is the major cause of mortality in intensive care units worldwide. Structural information on peptides bound to LPS can lead to the development of more effective endotoxin neutralizers. Short linear antimicrobial and endotoxin-neutralizing peptide LF11, based on the human lactoferrin, binds to LPS, inducing a peptide fold with a "T-shaped" arrangement of a hydrophobic core and two clusters of basic residues that match the distance between the two phosphate groups of LPS. Side chain arrangement of LF11 bound to LPS extends the previously proposed LPS binding pattern, emphasizing the importance of both electrostatic and hydrophobic interactions in a defined geometric arrangement. In anionic micelles, the LF11 forms amphipathic conformation with a smaller hydrophobic core than in LPS, whereas in zwitterionic micelles, the structure is even less defined. Protection of tryptophan fluorescence quenching in the order SDS>LPS>DPC and hydrogen exchange protection indicates the decreasing extent of insertion of the N terminus and potential role of peptide plasticity in differentiation between bacterial and eukaryotic membranes.
PubMed: 15687491
DOI: 10.1074/jbc.M500266200
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

226707

數據於2024-10-30公開中

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