1XTN

crystal structure of CISK-PX domain with sulfates

1XTN の概要

• エントリーDOI: 10.2210/pdb1xtn/pdb
• 関連するPDBエントリー: 1XTE
• 分子名称: Serine/threonine-protein kinase Sgk3, SULFATE ION (3 entities in total)
• 機能のキーワード: cisk, px domain, transferase
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cytoplasmic vesicle : Q9ERE3
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29109.17

構造登録者

Xing, Y.,Liu, D.,Zhang, R.,Joachimiak, A.,Songyang, Z.,Xu, W. (登録日: 2004-10-22, 公開日: 2004-11-02, 最終更新日: 2023-08-23)

主引用文献

Xing, Y.,Liu, D.,Zhang, R.,Joachimiak, A.,Songyang, Z.,Xu, W.
Structural basis of membrane targeting by the Phox homology domain of cytokine-independent survival kinase (CISK-PX)
J.Biol.Chem., 279:30662-30669, 2004

PubMed Abstract: The cytokine-independent survival kinase (CISK) in the serum and glucocorticoid-regulated kinase family plays an important role in mediating cell growth and survival. N-terminal to its catalytic kinase domain, CISK contains a phox homology (PX) domain, a phosphoinositide-binding motif that directs the membrane localization of CISK and regulates CISK activity. We have determined the crystal structures of the mouse CISK-PX domain to unravel the structural basis of membrane targeting of CISK. In addition to the specific interactions conferred by the phosphoinositide-binding pocket, the structure suggests that a hydrophobic loop region and a hydrophilic beta-turn contribute to the interactions with the membrane. Furthermore, biochemical studies reveal that CISK-PX dimerizes in the presence of the linker between the PX domain and kinase domain, suggesting a multivalent mechanism in membrane localization of CISK.

PubMed: 15126499
DOI: 10.1074/jbc.M404107200

実験手法

X-RAY DIFFRACTION (2.2 Å)