1XT6

S35C Flavodoxin Mutant in the semiquinone state

Summary for 1XT6

Entry DOI	10.2210/pdb1xt6/pdb
Descriptor	Flavodoxin, FLAVIN MONONUCLEOTIDE (3 entities in total)
Functional Keywords	protein, flavodoxin, mutant, s35c, electron transport
Biological source	Desulfovibrio vulgaris subsp. vulgaris
Total number of polymer chains	1
Total formula weight	16150.52
Authors	Artali, R.,Marchini, N.,Meneghetti, F.,Cavazzini, D.,Cassetta, A.,Sassone, C.,Bombieri, G.,Rossi, G.L.,Gilardi, G. (deposition date: 2004-10-21, release date: 2004-12-21, Last modification date: 2024-02-14)
Primary citation	Artali, R.,Marchini, N.,Meneghetti, F.,Cavazzini, D.,Cassetta, A.,Sassone, C. Structure of S35C flavodoxin mutant from Desulfovibrio vulgaris in the semiquinone state. Acta Crystallogr.,Sect.D, 61:481-484, 2005 Cited by PubMed Abstract: The crystallographic structure of an engineered flavodoxin mutant from Desulfovibrio vulgaris has been analysed. Site-directed mutagenesis was used to substitute serine 35 with a cysteine to provide a possible covalent linkage. The crystal structure of the semiquinone form of this mutant is similar to the corresponding oxidation state of the wild-type flavodoxin. Analysis of the structural changes reveals the interaction between N(5)H of the flavin and the carbonyl O atom of Gly61 to be critical for modulation of the electrochemical properties of the protein. PubMed: 15805604 DOI: 10.1107/S0907444905001502 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.8 Å)

Structure validation