1XS0

Structure of the E. coli Ivy protein

1XS0 の概要

• エントリーDOI: 10.2210/pdb1xs0/pdb
• 関連するPDBエントリー: 1GPQ 1HKE
• 分子名称: Inhibitor of vertebrate lysozyme (2 entities in total)
• 機能のキーワード: alpha beta fold, dimer, hydrolase inhibitor
• 由来する生物種: Escherichia coli
• 細胞内の位置: Periplasm: P45502
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 45182.50

構造登録者

Abergel, C.,Monchois, V.,Byrn, D.,Lazzaroni, J.C.,Claverie, J.M. (登録日: 2004-10-18, 公開日: 2004-11-02, 最終更新日: 2024-10-30)

主引用文献

Abergel, C.,Monchois, V.,Byrne, D.,Chenivesse, S.,Lembo, F.,Lazzaroni, J.C.,Claverie, J.M.
Structure and evolution of the Ivy protein family, unexpected lysozyme inhibitors in Gram-negative bacteria.
Proc.Natl.Acad.Sci.USA, 104:6394-6399, 2007

PubMed Abstract: Part of an ancestral bactericidal system, vertebrate C-type lysozyme targets the peptidoglycan moiety of bacterial cell walls. We report the crystal structure of a protein inhibitor of C-type lysozyme, the Escherichia coli Ivy protein, alone and in complex with hen egg white lysozyme. Ivy exhibits a novel fold in which a protruding five-residue loop appears essential to its inhibitory effect. This feature guided the identification of Ivy orthologues in other Gram-negative bacteria. The structure of the evolutionary distant Pseudomonas aeruginosa Ivy orthologue was also determined in complex with hen egg white lysozyme, and its antilysozyme activity was confirmed. Ivy expression protects porous cell-wall E. coli mutants from the lytic effect of lysozyme, suggesting that it is a response against the permeabilizing effects of the innate vertebrate immune system. As such, Ivy acts as a virulence factor for a number of Gram-negative bacteria-infecting vertebrates.

PubMed: 17405861
DOI: 10.1073/pnas.0611019104

実験手法

X-RAY DIFFRACTION (1.58 Å)