1XQM

Variations on the GFP chromophore scaffold: A fragmented 5-membered heterocycle revealed in the 2.1A crystal structure of a non-fluorescent chromoprotein

1XQM の概要

• エントリーDOI: 10.2210/pdb1xqm/pdb
• 分子名称: kindling fluorescent protein, ACETIC ACID (3 entities in total)
• 機能のキーワード: luminescent protein
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25898.54

構造登録者

Wilmann, P.G.,Petersen, J.,Devenish, R.J.,Prescott, M.,Rossjohn, J. (登録日: 2004-10-13, 公開日: 2004-11-16, 最終更新日: 2026-03-18)

主引用文献

Wilmann, P.G.,Petersen, J.,Devenish, R.J.,Prescott, M.,Rossjohn, J.
Variations on the GFP chromophore: A polypeptide fragmentation within the chromophore revealed in the 2.1-A crystal structure of a nonfluorescent chromoprotein from Anemonia sulcata
J.Biol.Chem., 280:2401-2404, 2005

PubMed Abstract: We have determined to 2.1 A resolution the crystal structure of a dark state, kindling fluorescent protein isolated from the sea anemone, Anemonia sulcata. The chromophore sequence Met(63)-Tyr(64)-Gly(65) of the A. sulcata chromoprotein was previously proposed to comprise a 6-membered pyrazine-type heterocycle (Martynov, V. I., Savitsky, A. P., Martynova, N. Y., Savitsky, P. A., Lukyanov, K. A., and Lukyanov, S. A. (2001) J. Biol. Chem. 276, 21012-21016). However, our crystallographic data revealed the chromophore to comprise a 5-membered p-hydroxybenzylideneimidazolinone moiety that adopts a non-coplanar trans conformation within the interior of the GFP beta-can fold. Unexpectedly, fragmentation of the polypeptide was found to occur within the chromophore moiety, at the bond between Cys(62C) and Met(63N1.) Our structural data reveal that fragmentation of the chromophore represents an intrinsic, autocatalytic step toward the formation of the mature chromophore within the specific GFP-like proteins.

PubMed: 15542608
DOI: 10.1074/jbc.C400484200

実験手法

X-RAY DIFFRACTION (2.1 Å)