1XOK

crystal structure of alfalfa mosaic virus RNA 3'UTR in complex with coat protein N terminal peptide

1XOK の概要

• エントリーDOI: 10.2210/pdb1xok/pdb
• 分子名称: alfalfa mosaic virus RNA 3' UTR, Coat protein, BROMIDE ION, ... (5 entities in total)
• 機能のキーワード: protein-rna complex, alpha helix, atypical rna duplex, viral protein-rna complex, viral protein/rna
• 細胞内の位置: Virion (Potential): P24264
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 18060.80

構造登録者

Guogas, L.M.,Filman, D.J.,Hogle, J.M.,Gehrke, L. (登録日: 2004-10-06, 公開日: 2005-01-04, 最終更新日: 2024-02-14)

主引用文献

Guogas, L.M.,Filman, D.J.,Hogle, J.M.,Gehrke, L.
Cofolding organizes alfalfa mosaic virus RNA and coat protein for replication.
Science, 306:2108-2111, 2004

PubMed Abstract: Alfalfa mosaic virus genomic RNAs are infectious only when the viral coat protein binds to the RNA 3' termini. The crystal structure of an alfalfa mosaic virus RNA-peptide complex reveals that conserved AUGC repeats and Pro-Thr-x-Arg-Ser-x-x-Tyr coat protein amino acids cofold upon interacting. Alternating AUGC residues have opposite orientation, and they base pair in different adjacent duplexes. Localized RNA backbone reversals stabilized by arginine-guanine interactions place the adenosines and guanines in reverse order in the duplex. The results suggest that a uniform, organized 3' conformation, similar to that found on viral RNAs with transfer RNA-like ends, may be essential for replication.

PubMed: 15604410
DOI: 10.1126/science.1103399

実験手法

X-RAY DIFFRACTION (3 Å)