1XKI
Crystal structure of human tear lipocalin/von Ebners gland protein
Summary for 1XKI
| Entry DOI | 10.2210/pdb1xki/pdb |
| Descriptor | Von Ebner's gland protein, ZINC ION, CHLORIDE ION, ... (4 entities in total) |
| Functional Keywords | beta barrel, ligand binding protein, transport protein |
| Biological source | Homo sapiens (human) |
| Cellular location | Secreted: P31025 |
| Total number of polymer chains | 1 |
| Total formula weight | 18216.31 |
| Authors | Breustedt, D.A.,Korndoerfer, I.P.,Redl, B.,Skerra, A. (deposition date: 2004-09-29, release date: 2004-10-19, Last modification date: 2024-05-29) |
| Primary citation | Breustedt, D.A.,Korndoerfer, I.P.,Redl, B.,Skerra, A. The 1.8-A crystal structure of human tear lipocalin reveals an extended branched cavity with capacity for multiple ligands J.Biol.Chem., 280:484-493, 2005 Cited by PubMed Abstract: In contrast with earlier assumptions, which classified human tear lipocalin (Tlc) as an outlier member of the lipocalin protein family, the 1.8-A resolution crystal structure of the recombinant apoprotein confirms the typical eight-stranded antiparallel beta-barrel architecture with an alpha-helix attached to it. The fold of Tlc most closely resembles the bovine dander allergen Bos d 2, a well characterized prototypic lipocalin, but also reveals similarity with beta-lactoglobulin. However, compared with other lipocalin structures Tlc exhibits an extremely wide ligand pocket, whose entrance is formed by four partially disordered loops. The cavity deeply extends into the beta-barrel structure, where it ends in two distinct lobes. This unusual structural feature explains the known promiscuity of Tlc for various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampin and even to microbial siderophores. Notably, earlier findings of biological activity as a thiol protease inhibitor have no correspondence in the three-dimensional structure of Tlc, rather it appears that its proteolytic fragments could be responsible for this phenomenon. Hence, the present structural analysis sheds new light on the ligand binding activity of this functionally obscure but abundant human lipocalin. PubMed: 15489503DOI: 10.1074/jbc.M410466200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.8 Å) |
Structure validation
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