1XJ2

CO-bound structure of bjFixLH

1XJ2 の概要

• エントリーDOI: 10.2210/pdb1xj2/pdb
• 関連するPDBエントリー: 1XJ3 1XJ4 1XJ6
• 分子名称: Sensor protein fixL, PROTOPORPHYRIN IX CONTAINING FE, CARBON MONOXIDE, ... (4 entities in total)
• 機能のキーワード: pas domain; heme; oxygen sensor; carbon monoxide, signaling protein, transferase
• 由来する生物種: Bradyrhizobium japonicum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13746.20

構造登録者

Key, J.,Moffat, K. (登録日: 2004-09-22, 公開日: 2005-03-29, 最終更新日: 2023-08-23)

主引用文献

Key, J.,Moffat, K.
Crystal Structures of Deoxy and CO-Bound bjFixLH Reveal Details of Ligand Recognition and Signaling
Biochemistry, 44:4627-4635, 2005

PubMed Abstract: Rhizobia directly regulate the expression of genes required for symbiotic nitrogen fixation in response to oxygen concentration via the sensor protein FixL. The N-terminal PAS domain of FixL contains a histidine-coordinated heme and regulates the activity of its effector domain, a C-terminal histidine kinase, in response to binding of oxygen and other ligands at the heme. To further investigate ligand-induced inhibition of FixL, we have determined the crystal structures of the heme domain in both the deoxy state and bound to carbon monoxide, a weak inhibitor of FixL kinase activity. Structures collected at room temperature are presented in each state from two crystallographic space groups at 1.8 and 2 A resolution. These structures reveal displacement of the residues of the H(beta) and I(beta) strands by Leu236 upon CO binding, and this structural change propagates more than 15 A to a region of the structure implicated in signal transduction in PAS proteins. Displacement of residues Ile215, Ile216, and Gly217 in the FG loop is also evident, accompanied by the movement of heme propionate 6 upon change in iron ligation. CO binding increases the temperature factors in the FG loop of the protein and disorders the side chain of Arg206, a conserved residue involved in the FG loop switch mechanism. We relate these results to structural changes in other PAS sensor domains and their involvement in catalytic control.

PubMed: 15779889
DOI: 10.1021/bi047942r

実験手法

X-RAY DIFFRACTION (2 Å)