1XI2

Quinone Reductase 2 in Complex with Cancer Prodrug CB1954

Summary for 1XI2

• Entry DOI: 10.2210/pdb1xi2/pdb
• Related: 1QR2 2QR2
• Descriptor: NRH dehydrogenase [quinone] 2, ZINC ION, FLAVIN-ADENINE DINUCLEOTIDE, ... (5 entities in total)
• Functional Keywords: qr2, cb1954, oxidoreductase
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 53970.37

Authors

Fu, Y.,Buryanovskyy, L.,Zhang, Z. (deposition date: 2004-09-21, release date: 2005-08-30, Last modification date: 2024-02-14)

Primary citation

Fu, Y.,Buryanovskyy, L.,Zhang, Z.
Crystal structure of quinone reductase 2 in complex with cancer prodrug CB1954
Biochem.Biophys.Res.Commun., 336:332-338, 2005

PubMed Abstract: CB1954 is a cancer pro-drug that can be activated through reduction by Escherichia coli nitro-reductases and quinone reductases. Human quinone reductase 2 is very efficient in the activation of CB1954, approximately 3000 times more efficient than human QR1 in terms of k(cat)/K(m). We have solved the three-dimensional structure of QR2 in complex with CB1954 to a nominal resolution of 1.5A. The complex structure indicates the essentiality of the two nitro groups: one nitro group forms hydrogen bonds with the side-chain of Asn161 of QR2 to hold the other nitro group in position for the reduction. We further conclude that residue 161, an Asn in QR2 and a His in QR1, is critical in differentiating the substrate specificities of these two enzymes. Mutation of Asn161 to His161 in QR2 resulted in the total loss of the enzymatic activity towards activation of CB1954, whereas the rates of reduction towards menadione are not altered.

PubMed: 16129418
DOI: 10.1016/j.bbrc.2005.08.081

Experimental method

X-RAY DIFFRACTION (1.5 Å)