1XI0

X-ray crystal structure of wild-type Xerocomus chrysenteron lectin XCL

1XI0 の概要

• エントリーDOI: 10.2210/pdb1xi0/pdb
• 関連するPDBエントリー: 1X99
• 分子名称: lectin (2 entities in total)
• 機能のキーワード: beta sandwich, greek key motif, sugar binding protein
• 由来する生物種: Xerocomus chrysenteron
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32063.62

構造登録者

Birck, C.,Damian, L.,Marty-Detraves, C.,Lougarre, A.,Schulze-Briese, C.,Koehl, P.,Fournier, D.,Paquereau, L.,Samama, J.P. (登録日: 2004-09-21, 公開日: 2005-08-30, 最終更新日: 2023-10-25)

主引用文献

Birck, C.,Damian, L.,Marty-Detraves, C.,Lougarre, A.,Schulze-Briese, C.,Koehl, P.,Fournier, D.,Paquereau, L.,Samama, J.P.
A new lectin family with structure similarity to actinoporins revealed by the crystal structure of Xerocomus chrysenteron lectin XCL
J.Mol.Biol., 344:1409-1420, 2004

PubMed Abstract: A newly defined family of fungal lectins displays no significant sequence similarity to any protein in the databases. These proteins, made of about 140 amino acid residues, have sequence identities ranging from 38% to 65% and share binding specificity to N-acetyl galactosamine. One member of this family, the lectin XCL from Xerocomus chrysenteron, induces drastic changes in the actin cytoskeleton after sugar binding at the cell surface and internalization, and has potent insecticidal activity. The crystal structure of XCL to 1.4 A resolution reveals the architecture of this new lectin family. The fold of the protein is not related to any of the several lectin folds documented so far. Unexpectedly, the structure similarity is significant with actinoporins, a family of pore-forming toxins. The specific structural features and sequence signatures in each protein family suggest a potential sugar binding site in XCL and a possible evolutionary relationship between these proteins. Finally, the tetrameric assembly of XCL reveals a complex network of protomer-protomer interfaces and generates a large, hydrated cavity of 1000 A3, which may become accessible to larger solutes after a small conformational change of the protein.

PubMed: 15561152
DOI: 10.1016/j.jmb.2004.10.007

実験手法

X-RAY DIFFRACTION (2 Å)