1XBI

High resolution structure of Methanocaldococcus jannaschii L7AE

1XBI の概要

• エントリーDOI: 10.2210/pdb1xbi/pdb
• 分子名称: 50S ribosomal protein L7Ae, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID (3 entities in total)
• 機能のキーワード: alpha-beta-alpha sandwich fold, rna binding protein-structural protein complex, rna binding protein/structural protein
• 由来する生物種: Methanocaldococcus jannaschii
• 細胞内の位置: Cytoplasm : P54066
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13224.42

構造登録者

Brown II, B.A.,Suryadi, J.,Lieberman, D.V.,Tran, E.J.,Maxwell, E.S. (登録日: 2004-08-30, 公開日: 2005-08-09, 最終更新日: 2023-08-23)

主引用文献

Suryadi, J.,Tran, E.J.,Maxwell, E.S.,Brown II, B.A.
The Crystal Structure of the Methanocaldococcus jannaschii Multifunctional L7Ae RNA-Binding Protein Reveals an Induced-Fit Interaction with the Box C/D RNAs.
Biochemistry, 44:9657-9672, 2005

PubMed Abstract: Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal, box H/ACA, and box C/D sRNAs. The crystal structure of Methanocaldococcus jannaschii L7Ae has been determined to 1.45 A, and L7Ae's amino acid composition, evolutionary conservation, functional characteristics, and structural details have been analyzed. Comparison of the L7Ae structure to those of a number of related proteins with diverse functions has revealed significant structural homology which suggests that this protein fold is an ancient RNA-binding motif. Notably, the free M. jannaschii L7Ae structure is essentially identical to that with RNA bound, suggesting that RNA binding occurs through an induced-fit interaction. Circular dichroism experiments show that box C/D and C'/D' RNA motifs undergo conformational changes when magnesium or the L7Ae protein is added, corroborating the induced-fit model for L7Ae-box C/D RNA interactions.

PubMed: 16008351
DOI: 10.1021/bi050568q

実験手法

X-RAY DIFFRACTION (1.45 Å)