1X84

IPP isomerase (wt) reacted with (S)-bromohydrine of IPP

1X84 の概要

• エントリーDOI: 10.2210/pdb1x84/pdb
• 関連するPDBエントリー: 1PPV 1X83
• 分子名称: Isopentenyl-diphosphate delta-isomerase, MANGANESE (II) ION, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: isomerase, complex
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: Q46822
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 43791.03

構造登録者

Wouters, J.,Oldfield, E. (登録日: 2004-08-17, 公開日: 2005-01-25, 最終更新日: 2024-12-25)

主引用文献

Wouters, J.,Yin, F.,Song, Y.,Zhang, Y.,Oudjama, Y.,Stalon, V.,Droogmans, L.,Morita, C.T.,Oldfield, E.
A Crystallographic Investigation of Phosphoantigen Binding to Isopentenyl Pyrophosphate/Dimethylallyl Pyrophosphate Isomerase
J.Am.Chem.Soc., 127:536-537, 2005

PubMed Abstract: We report the crystallographic structures of the potent phosphoantigens Phosphostim (the bromohydrin of isopentenyl pyrophosphate) and E-4-hydroxy-3-methyl-but-2-enyl pyrophosphate bound to the mevalonate pathway enzyme isopentenyl pyrophosphate/dimethylallyl pyrophosphate isomerase (IPPI). Racemic Phosphostim forms covalent complexes with IPPI: a 4-thioether with C67 and a 4-ester with E116. Only the E116 ester forms with the chiral species, S-Phosphostim, with the w.t. enzyme, while the C67 thioether forms with a mutant Y104F IPPI. The potent phosphoantigen HMBPP also binds to IPPI, but is only a weak ( approximately 50 muM) inhibitor. These results strongly support an SN2 reaction for inhibition of IPPI by Phosphostim, in contrast to the SN1 or concerted type of reaction found with epoxide inhibitors, which react at C-3, and are of general interest in the context of the development of novel mevalonate pathway inhibitors. They also provide clues as to the nature of the binding site of synthetic phosphoantigens in gammadelta T cell activation. In particular, both bromohydrin and epoxy phosphoantigens are potent, irreversible inhibitors of IPPI while HMBPP is only a weak inhibitor, ruling out an IPPI or IPPI-like target for HMBPP in gammadelta T cell activation.

PubMed: 15643873
DOI: 10.1021/ja040207i

実験手法

X-RAY DIFFRACTION (1.78 Å)