1X77

Crystal structure of a NAD(P)H-dependent FMN reductase complexed with FMN

1X77 の概要

• エントリーDOI: 10.2210/pdb1x77/pdb
• 関連するPDBエントリー: 1RTT
• 分子名称: conserved hypothetical protein, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: structural genomics, protein structure initiative (psi), fmn reductase, fmn bound, new york sgx research center for structural genomics, nysgxrc, unknown function
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 43150.37

構造登録者

Agarwal, R.,Swaminathan, S.,Burley, S.K.,New York SGX Research Center for Structural Genomics (NYSGXRC) (登録日: 2004-08-13, 公開日: 2004-08-24, 最終更新日: 2023-08-23)

主引用文献

Agarwal, R.,Bonanno, J.B.,Burley, S.K.,Swaminathan, S.
Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal.
ACTA CRYSTALLOGR.,SECT.D, 62:383-391, 2006

PubMed Abstract: The availability of high-intensity synchrotron facilities, technological advances in data-collection techniques and improved data-reduction and crystallographic software have ushered in a new era in high-throughput macromolecular crystallography. Here, the de novo automated crystal structure determination at 1.28 A resolution of an NAD(P)H-dependent FMN reductase flavoprotein from Pseudomonas aeruginosa PA01-derived protein Q9I4D4 using the anomalous signal from an unusually small number of S atoms is reported. Although this protein lacks the flavodoxin key fingerprint motif [(T/S)XTGXT], it has been confirmed to bind flavin mononucleotide and the binding site was identified via X-ray crystallography. This protein contains a novel flavin mononucleotide-binding site GSLRSGSYN, which has not been previously reported. Detailed statistics pertaining to sulfur phasing and other factors contributing to structure determination are discussed. Structural comparisons of the apoenzyme and the protein complexed with flavin mononucleotide show conformational changes on cofactor binding. NADPH-dependent activity has been confirmed with biochemical assays.

PubMed: 16552139
DOI: 10.1107/S0907444906001600

実験手法

X-RAY DIFFRACTION (2.7 Å)