1X46

Crystal structure of a hemoglobin component (TA-VII) from Tokunagayusurika akamusi

1X46 の概要

• エントリーDOI: 10.2210/pdb1x46/pdb
• 分子名称: hemoglobin component VII, PROTOPORPHYRIN IX CONTAINING FE (3 entities in total)
• 機能のキーワード: hemoglobin, diptera, tokunagayusurika akamusi, midge larva, oxygen storage-transport complex, oxygen storage/transport
• 由来する生物種: Tokunagayusurika akamusi
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17159.07

構造登録者

Kuwada, T.,Hasegawa, T.,Sato, S.,Sato, I.,Ishikawa, K.,Takagi, T.,Shishikura, F. (登録日: 2005-05-14, 公開日: 2005-05-24, 最終更新日: 2024-03-13)

主引用文献

Kuwada, T.,Hasegawa, T.,Sato, S.,Sato, I.,Ishikawa, K.,Takagi, T.,Shishikura, F.
Crystal structures of two hemoglobin components from the midge larva Propsilocerus akamusi (Orthocladiinae, Diptera).
Gene, 398:29-34, 2007

PubMed Abstract: The polymorphic components of hemoglobin (Hb) of the midge larva Propsilocerus akamusi were classified into two distinct types dependent on their spectroscopic properties, normal absorption (N) and low absorption (L). Analyses of the amino acid sequences of component VII (N-type Hb) and component V (L-type Hb) from P. akamusi indicated that one remarkable difference is the replacement of the distal histidine (His) with isoleucine (Ile) in component V. To clarify the structural differences between the two Hb components, we determined the crystal structures of components V and VII at resolutions of 1.64 A and 1.50 A, respectively. These crystal structures indicated a short additional helix comprising three amino acid residues at the C-terminal region in component V, and a typical globin fold including eight helices in component VII. Comparison of the heme regions of the Hb components suggests that the structural changes of the heme region in component V on ligation differ from that of usual Hb.

PubMed: 17590288
DOI: 10.1016/j.gene.2007.02.049

実験手法

X-RAY DIFFRACTION (1.5 Å)