1X3F

Crystal structure of the single-stranded DNA-binding protein from Mycobacterium SMEGMATIS

1X3F の概要

• エントリーDOI: 10.2210/pdb1x3f/pdb
• 関連するPDBエントリー: 1X3E 1X3G
• 分子名称: Single-strand binding protein, CADMIUM ION (3 entities in total)
• 機能のキーワード: oligonucleotide binding fold, dna-binding protein, dna binding protein
• 由来する生物種: Mycobacterium smegmatis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35067.02

構造登録者

Saikrishnan, K.,Manjunath, G.P.,Singh, P.,Jeyakanthan, J.,Dauter, Z.,Sekar, K.,Muniyappa, K.,Vijayan, M. (登録日: 2005-05-05, 公開日: 2005-08-15, 最終更新日: 2024-03-13)

主引用文献

Saikrishnan, K.,Manjunath, G.P.,Singh, P.,Jeyakanthan, J.,Dauter, Z.,Sekar, K.,Muniyappa, K.,Vijayan, M.
Structure of Mycobacterium smegmatis single-stranded DNA-binding protein and a comparative study involving homologus SSBs: biological implications of structural plasticity and variability in quaternary association.
Acta Crystallogr.,Sect.D, 61:1140-1148, 2005

PubMed Abstract: The structure of Mycobacterium smegmatis single-stranded DNA-binding protein (SSB) has been determined using three data sets collected from related crystals. The structure is similar to that of its homologue from Mycobacterium tuberculosis, indicating that the clamp arrangement that stabilizes the dimer and the ellipsoidal shape of the tetramer are characteristic features of mycobacterial SSBs. The central OB fold is conserved in mycobacterial SSBs as well as those from Escherichia coli, Deinococcus radiodurans and human mitochondria. However, the quaternary structure exhibits considerable variability. The observed plasticity of the subunit is related to this variability. The crystal structures and modelling provide a rationale for the variability. The strand involved in the clamp mechanism, which leads to higher stability of the tetramer, appears to occur in all high-G+C Gram-positive bacteria. The higher stability is perhaps required by these organisms. The mode of DNA binding of mycobacterial SSBs is different from that of E. coli SSB partly on account of the difference in the shape of the tetramers. Another difference between the two modes is that the former contains additional ionic interactions and is more susceptible to salt concentration.

PubMed: 16041080
DOI: 10.1107/S0907444905016896

実験手法

X-RAY DIFFRACTION (2.7 Å)