1X22

Solution structure of a novel moricin analogue, an antibacterial peptide from a lepidopteran insect, Spodoptera litura

1X22 の概要

• エントリーDOI: 10.2210/pdb1x22/pdb
• 分子名称: moricin (1 entity in total)
• 機能のキーワード: insect immunity, antibacterial peptide, moricin, purification, gene expression, solution structure, antibiotic
• 由来する生物種: Spodoptera litura
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4502.46

構造登録者

Oizumi, Y.,Hemmi, H.,Minami, M.,Asaoka, A.,Yamakawa, M. (登録日: 2005-04-18, 公開日: 2005-09-06, 最終更新日: 2024-05-01)

主引用文献

Oizumi, Y.,Hemmi, H.,Minami, M.,Asaoka, A.,Yamakawa, M.
Isolation, gene expression and solution structure of a novel moricin analogue, antibacterial peptide from a lepidopteran insect, Spodoptera litura
Biochim.Biophys.Acta, 1752:83-92, 2005

PubMed Abstract: An antibacterial peptide was isolated from a lepidopteran insect, Spodoptera litura. The molecular mass of this peptide was determined to be 4489.55 by matrix assisted laser desorption/ionization-time of flight mass (MALDI-TOF MS) spectrometry. The peptide consists of 42 amino acids and the sequence has 69-98% identity to those of moricin-related peptides, antibacterial peptides from lepidopetran insects. Thus, the peptide was designated S. litura (Sl) moricin. Sl moricin showed a broad antibacterial spectrum against Gram-positive and negative bacteria. Sl moricin gene was inducible by bacterial injection and expressed tissue-specifically in the fat body and hemocytes. Furthermore, the solution structure of Sl moricin was determined by two-dimensional (2D) 1H-nuclear magnetic resonance (NMR) spectroscopy and hybrid distance geometry-simulated annealing calculation. The tertiary structure revealed a long alpha-helix containing eight turns along nearly the full length of the peptide like that of moricin, confirming that Sl moricin is a new moricin-like antibacterial peptide. These results suggest that moricin is present not only in B. mori but also in other lepidopteran insects forming a gene family.

PubMed: 16115804
DOI: 10.1016/j.bbapap.2005.07.013

実験手法

SOLUTION NMR