1X0S

Crystal structure of the 13-cis isomer of bacteriorhodopsin

1X0S の概要

• エントリーDOI: 10.2210/pdb1x0s/pdb
• 関連するPDBエントリー: 1iw6 1X0I 1X0K
• 分子名称: Bacteriorhodopsin, beta-D-galactopyranose-(1-6)-alpha-D-mannopyranose-(1-2)-alpha-D-glucopyranose, SULFATE ION, ... (7 entities in total)
• 機能のキーワード: proton pump, retinal, memebrane protein, proton transport
• 由来する生物種: Halobacterium salinarum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31893.22

構造登録者

Nishikawa, T.,Murakami, M.,Kouyama, T. (登録日: 2005-03-28, 公開日: 2005-08-30, 最終更新日: 2024-10-23)

主引用文献

Nishikawa, T.,Murakami, M.,Kouyama, T.
Crystal structure of the 13-cis isomer of bacteriorhodopsin in the dark-adapted state.
J.Mol.Biol., 352:319-328, 2005

PubMed Abstract: The atomic structure of the trans isomer of bacteriorhodopsin was determined previously by using a 3D crystal belonging to the space group P622. Here, a structure is reported for another isomer with the 13-cis, 15-syn retinal in a dark-adapted crystal. Structural comparison of the two isomers indicates that retinal isomerization around the C13[double bond]C14 and the C15[double bond]N bonds is accompanied by noticeable displacements of a few residues in the vicinity of the retinal Schiff base and small re-arrangement of the hydrogen-bonding network in the proton release channel. On the other hand, aromatic residues surrounding the retinal polyene chain were found to scarcely move during the dark/light adaptation. This result suggests that variation in the structural rigidity within the retinal-binding pocket is one of the important factors ensuring the stereospecific isomerization of retinal.

PubMed: 16084526
DOI: 10.1016/j.jmb.2005.07.021

実験手法

X-RAY DIFFRACTION (2.5 Å)