1WZA

Crystal structure of alpha-amylase from H.orenii

1WZA の概要

• エントリーDOI: 10.2210/pdb1wza/pdb
• 関連するPDBエントリー: 1GVI 1SMA
• 分子名称: alpha-amylase A, CALCIUM ION (3 entities in total)
• 機能のキーワード: halothermothrix orenii, hydrolase, alpha amylase, halophilic, thermophilic
• 由来する生物種: Halothermothrix orenii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 56825.39

構造登録者

Sivakumar, N.,Swaminathan, K.,Li, N. (登録日: 2005-03-03, 公開日: 2006-04-11, 最終更新日: 2023-10-25)

主引用文献

Sivakumar, N.,Li, N.,Tang, J.W.,Patel, B.K.,Swaminathan, K.
Crystal structure of AmyA lacks acidic surface and provide insights into protein stability at poly-extreme condition.
Febs Lett., 580:2646-2652, 2006

PubMed Abstract: Here we report the first crystal structure of a protein, AmyA, a secretory alpha-amylase isolated from Halothermothrix orenii, which is both halophilic and thermophilic. The crystal structure was determined at 1.6 A resolution. AmyA lacks the conserved acidic surface, which is considered essential for protein stability at high salinity. Sedimentation velocity and CD experiments on AmyA reveal the formation of unique reversible poly-dispersed oligomers that show unusually high thermal stability. These studies provide valuable insight into the structural elements that contribute to the stability of AmyA at both physical and chemical extremes and their functional implications.

PubMed: 16647060
DOI: 10.1016/j.febslet.2006.04.017

実験手法

X-RAY DIFFRACTION (1.6 Å)