1WZ3

The crystal structure of plant ATG12

1WZ3 の概要

• エントリーDOI: 10.2210/pdb1wz3/pdb
• 分子名称: autophagy 12b (2 entities in total)
• 機能のキーワード: ubiquitin-fold, plant protein
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21045.80

構造登録者

Suzuki, N.N.,Yoshimoto, K.,Fujioka, Y.,Ohsumi, Y.,Inagaki, F. (登録日: 2005-02-22, 公開日: 2005-06-21, 最終更新日: 2024-03-13)

主引用文献

Suzuki, N.N.,Yoshimoto, K.,Fujioka, Y.,Ohsumi, Y.,Inagaki, F.
The crystal structure of plant ATG12 and its biological implication in autophagy.
Autophagy, 1:119-126, 2005

PubMed Abstract: Atg12 is a post-translational modifier that is activated and conjugated to its single target, Atg5, by a ubiquitin-like conjugation system. The Atg12-Atg5 conjugate is essential for autophagy, the bulk degradation process of cytoplasmic components by the vacuolar/lysosomal system. Here, we demonstrate that the Atg12 conjugation system exists in Arabidopsis and is essential for plant autophagy as well as in yeast and mammals. We also report the crystal structure of Arabidopsis thaliana (At) ATG12 at 1.8 A resolution. Despite no obvious sequence homology with ubiquitin, the structure of AtATG12 shows a ubiquitin fold strikingly similar to those of mammalian homologs of Atg8, the other ubiquitin-like modifier essential for autophagy, which is conjugated to phosphatidylethanolamine. Two types of hydrophobic patches are present on the surface of AtATG12: one is conserved in both Atg12 and Atg8 orthologs, while the other is unique to Atg12 orthologs. Considering that they share Atg7 as an E1-like enzyme, we suggest that the first hydrophobic patch is responsible for the conjugation reaction, while the latter is involved in Atg12-specific functions.

PubMed: 16874047
DOI: 10.4161/auto.1.2.1859

実験手法

X-RAY DIFFRACTION (1.8 Å)