1WU6

Crystal structure of reducing-end-xylose releasing exo-oligoxylanase E70A mutant complexed with xylobiose

1WU6 の概要

• エントリーDOI: 10.2210/pdb1wu6/pdb
• 関連するPDBエントリー: 1WU4 1WU5
• 関連するBIRD辞書のPRD_ID: PRD_900116
• 分子名称: xylanase Y, beta-D-xylopyranose-(1-4)-beta-D-xylopyranose, NICKEL (II) ION, ... (5 entities in total)
• 機能のキーワード: (alpla/alpha)6 barrel, glycoside hydrolase family 8, hydrolase
• 由来する生物種: Bacillus halodurans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46595.89

構造登録者

Fushinobu, S.,Hidaka, M.,Honda, Y.,Wakagi, T.,Shoun, H.,Kitaoka, M. (登録日: 2004-12-01, 公開日: 2005-02-22, 最終更新日: 2023-10-25)

主引用文献

Fushinobu, S.,Hidaka, M.,Honda, Y.,Wakagi, T.,Shoun, H.,Kitaoka, M.
Structural Basis for the Specificity of the Reducing End Xylose-releasing Exo-oligoxylanase from Bacillus halodurans C-125
J.Biol.Chem., 280:17180-17186, 2005

PubMed Abstract: Reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125 (Rex) hydrolyzes xylooligosaccharides whose degree of polymerization is greater than or equal to 3, releasing the xylose unit at the reducing end. It is a unique exo-type glycoside hydrolase that recognizes the xylose unit at the reducing end in a very strict manner, even discriminating the beta-anomeric hydroxyl configuration from the alpha-anomer or 1-deoxyxylose. We have determined the crystal structures of Rex in unliganded and complex forms at 1.35-2.20-A resolution and revealed the structural aspects of its three subsites ranging from -2 to +1. The structure of Rex was compared with those of endo-type enzymes in glycoside hydrolase subfamily 8a (GH-8a). The catalytic machinery of Rex is basically conserved with other GH-8a enzymes. However, subsite +2 is blocked by a barrier formed by a kink in the loop before helix alpha10. His-319 in this loop forms a direct hydrogen bond with the beta-hydroxyl of xylose at subsite +1, contributing to the specific recognition of anomers at the reducing end.

PubMed: 15718242
DOI: 10.1074/jbc.M413693200

実験手法

X-RAY DIFFRACTION (1.45 Å)