1WTS

HELIX 45 (16S RRNA) FROM B. STEAROTHERMOPHILUS, NMR, MINIMIZED AVERAGE STRUCTURE

Summary for 1WTS

• Entry DOI: 10.2210/pdb1wts/pdb
• Descriptor: RNA (5'-R(*GP*GP*AP*CP*CP*2MGP*GP*MA6P*MA6P*GP*GP*UP*CP*C)-3') (1 entity in total)
• Functional Keywords: ribonucleic acid, ribosome, rna, 16s, modified nucleotides, tetraloop
• Total number of polymer chains: 1
• Total formula weight: 4610.92

Authors

Rife, J.P.,Moore, P.B. (deposition date: 1998-06-26, release date: 1999-01-27, Last modification date: 2024-05-22)

Primary citation

Rife, J.P.,Moore, P.B.
The structure of a methylated tetraloop in 16S ribosomal RNA.
Structure, 6:747-756, 1998

PubMed Abstract: Ribosomal RNAs contain many modified nucleotides. The functions of these nucleotides are poorly understood and few of them are strongly conserved. The final stem loop in 16S-like rRNAs is an exception in both regards. In both prokaryotes and eukaryotes, the tetranucleotide loop that caps the 3'-terminal stem contains two N6, N6-dimethyladenosine residues. The sequence and pattern of methylation are conserved within the loop, and there is evidence that these methylated nucleotides play an important role in subunit association and the initiation of protein synthesis. Because of the integral role that helix 45 plays in ribosome function, it is important to know what consequences these methylated nucleotides have on its structure.

PubMed: 9655826
DOI: 10.1016/S0969-2126(98)00076-8

Experimental method

SOLUTION NMR