1WSW

Low Temperature (100K) Crystal Structure Of Flavodoxin Mutant S64C, dimer, semiquinone state

1WSW の概要

• エントリーDOI: 10.2210/pdb1wsw/pdb
• 関連するPDBエントリー: 1WSB 1XYV 1XYY
• 分子名称: FLAVODOXIN, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: flavodoxin, s64c mutant, dimer, semireduced form, electron transport
• 由来する生物種: Desulfovibrio vulgaris
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16281.71

構造登録者

Artali, R.,Marchini, N.,Meneghetti, F.,Cavazzini, D.,Bombieri, G.,Rossi, G.L.,Gilardi, G. (登録日: 2004-11-12, 公開日: 2004-12-07, 最終更新日: 2024-11-06)

主引用文献

Fantuzzi, A.,Artali, R.,Bombieri, G.,Marchini, N.,Meneghetti, F.,Gilardi, G.,Sadeghi, S.J.,Cavazzini, D.,Rossi, G.L.
Redox properties and crystal structures of a Desulfovibrio vulgaris flavodoxin mutant in the monomeric and homodimeric forms.
Biochim.Biophys.Acta, 1794:496-505, 2009

PubMed Abstract: The mutant S64C of the short-chain flavodoxin from Desulfovibrio vulgaris has been designed to introduce an accessible and reactive group on the protein surface. Crystals have been obtained of both the monomeric and homodimeric forms of the protein, with the cofactor FMN in either the oxidized or the one electron-reduced (semiquinone) state, and the structures have been determined to high resolution. The redox properties of the different species have been investigated and the variations observed with respect to wild type have been related to the structural changes induced by the mutation and S-S bridge formation.

PubMed: 19118653
DOI: 10.1016/j.bbapap.2008.11.026

実験手法

X-RAY DIFFRACTION (1.69 Å)