1WRN

Metal Ion dependency of the antiterminator protein, HutP, for binding to the terminator region of hut mRNA- A structural basis

1WRN の概要

• エントリーDOI: 10.2210/pdb1wrn/pdb
• 関連するPDBエントリー: 1VEA 1WMQ 1WPS 1WPT 1WPU 1WPV 1WRO 1WRQ
• 分子名称: Hut operon positive regulatory protein, MANGANESE (II) ION, HISTIDINE, ... (5 entities in total)
• 機能のキーワード: hutp, rna binding protein, antitermination, l-histidine, metal ions, conformational change
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 49574.09

構造登録者

Kumarevel, T.,Mizuno, H.,Kumar, P.K.R. (登録日: 2004-10-25, 公開日: 2005-08-30, 最終更新日: 2024-05-29)

主引用文献

Kumarevel, T.,Mizuno, H.,Kumar, P.K.R.
Characterization of the metal ion binding site in the anti-terminator protein, HutP, of Bacillus subtilis
Nucleic Acids Res., 33:5494-5502, 2005

PubMed Abstract: HutP is an RNA-binding protein that regulates the expression of the histidine utilization (hut) operon in Bacillus subtilis, by binding to cis-acting regulatory sequences on hut mRNA. It requires L-histidine and an Mg2+ ion for binding to the specific sequence within the hut mRNA. In the present study, we show that several divalent cations can mediate the HutP-RNA interactions. The best divalent cations were Mn2+, Zn2+ and Cd2+, followed by Mg2+, Co2+ and Ni2+, while Cu2+, Yb2+ and Hg2+ were ineffective. In the HutP-RNA interactions, divalent cations cannot be replaced by monovalent cations, suggesting that a divalent metal ion is required for mediating the protein-RNA interactions. To clarify their importance, we have crystallized HutP in the presence of three different metal ions (Mg2+, Mn2+ and Ba2+), which revealed the importance of the metal ion binding site. Furthermore, these analyses clearly demonstrated how the metal ions cause the structural rearrangements that are required for the hut mRNA recognition.

PubMed: 16192572
DOI: 10.1093/nar/gki868

実験手法

X-RAY DIFFRACTION (2.3 Å)