1WR1

The complex structure of Dsk2p UBA with ubiquitin

1WR1 の概要

• エントリーDOI: 10.2210/pdb1wr1/pdb
• 関連するPDBエントリー: 1D3Z
• 分子名称: Ubiquitin, Ubiquitin-like protein DSK2 (2 entities in total)
• 機能のキーワード: uba domain, uba-ubiquitin complex, dsk2, signaling protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 14750.51

構造登録者

Ohno, A.,Jee, J.G.,Fujiwara, K.,Tenno, T.,Goda, N.,Tochio, H.,Hiroaki, H.,kobayashi, H.,Shirakawa, M. (登録日: 2004-10-08, 公開日: 2005-04-19, 最終更新日: 2023-09-27)

主引用文献

Ohno, A.,Jee, J.,Fujiwara, K.,Tenno, T.,Goda, N.,Tochio, H.,Kobayashi, H.,Hiroaki, H.,Shirakawa, M.
Structure of the UBA domain of Dsk2p in complex with ubiquitin molecular determinants for ubiquitin recognition.
Structure, 13:521-532, 2005

PubMed Abstract: The ubiquitin-associated (UBA) domain is one of the most frequently occurring motifs that recognize ubiquitin tags. Dsk2p, a UBA-containing protein from Saccharomyces cerevisiae, is involved in the ubiquitin-proteasome proteolytic pathway and has been implicated in spindle pole duplication. Here we present the solution structure of the UBA domain of Dsk2p (Dsk2(UBA)) in complex with ubiquitin. The structure reveals that the UBA domain uses a mode of ubiquitin recognition that is similar to that of the CUE domain, another ubiquitin binding motif that shares low sequence homology but high structural similarity with UBA domains. These two domains, as well as the structurally unrelated ubiquitin binding motif UIM, provide a common, crucial recognition site for ubiquitin, comprising a hydrogen-bonding acceptor for the amide group of Gly-47, and a methyl group that packs against the hydrophobic pocket of ubiquitin formed by Leu-8, Ile-44, His-68, and Val-70.

PubMed: 15837191
DOI: 10.1016/j.str.2005.01.011

実験手法

SOLUTION NMR