1WNE

Foot and Mouth Disease Virus RNA-dependent RNA polymerase in complex with a template-primer RNA

1WNE の概要

• エントリーDOI: 10.2210/pdb1wne/pdb
• 関連するPDBエントリー: 1U09
• 分子名称: 5'-R(*CP*AP*UP*GP*GP*GP*CP*C)-3', 5'-R(*GP*GP*CP*CP*C)-3', RNA-dependent RNA polymerase, ... (5 entities in total)
• 機能のキーワード: protein-dna complex, foot and mouth disease virus, rna-dependent rna polymerase, transferase-rna complex, transferase/rna
• 由来する生物種: Foot-and-mouth disease virus
• 細胞内の位置: Picornain 3C: Host cytoplasm (By similarity): Q9QCE4
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 57613.57

構造登録者

Ferrer-Orta, C.,Arias, A.,Perez-Luque, R.,Escarmis, C.,Domingo, E.,Verdaguer, N. (登録日: 2004-07-31, 公開日: 2004-08-31, 最終更新日: 2023-10-25)

主引用文献

Ferrer-Orta, C.,Arias, A.,Perez-Luque, R.,Escarmis, C.,Domingo, E.,Verdaguer, N.
Structure of Foot-and-Mouth Disease Virus RNA-dependent RNA Polymerase and Its Complex with a Template-Primer RNA
J.Biol.Chem., 279:47212-47221, 2004

PubMed Abstract: Genome replication in picornaviruses is catalyzed by a virally encoded RNA-dependent RNA polymerase, termed 3D. The enzyme performs this operation, together with other viral and probably host proteins, in the cytoplasm of their host cells. The crystal structure of the 3D polymerase of foot-and-mouth disease virus, one of the most important animal pathogens, has been determined unliganded and bound to a template-primer RNA decanucleotide. The enzyme folds in the characteristic fingers, palm and thumb subdomains, with the presence of an NH2-terminal segment that encircles the active site. In the complex, several conserved amino acid side chains bind to the template-primer, likely mediating the initiation of RNA synthesis. The structure provides essential information for studies on RNA replication and the design of antiviral compounds.

PubMed: 15294895
DOI: 10.1074/jbc.M405465200

実験手法

X-RAY DIFFRACTION (3 Å)