1WMB

Crystal structure of NAD dependent D-3-hydroxybutylate dehydrogenase

1WMB の概要

• エントリーDOI: 10.2210/pdb1wmb/pdb
• 分子名称: D(-)-3-hydroxybutyrate dehydrogenase, MAGNESIUM ION, CACODYLATE ION, ... (4 entities in total)
• 機能のキーワード: nad, nadh, short chain dehydrogenase, oxidoreductase
• 由来する生物種: Pseudomonas fragi
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53747.42

構造登録者

Ito, K.,Nakajima, Y.,Ichihara, E.,Ogawa, K.,Yoshimoto, T. (登録日: 2004-07-06, 公開日: 2005-09-06, 最終更新日: 2024-03-13)

主引用文献

Ito, K.,Nakajima, Y.,Ichihara, E.,Ogawa, K.,Katayama, N.,Nakashima, K.,Yoshimoto, T.
d-3-Hydroxybutyrate Dehydrogenase from Pseudomonas fragi: Molecular Cloning of the Enzyme Gene and Crystal Structure of the Enzyme
J.Mol.Biol., 355:722-733, 2006

PubMed Abstract: The gene coding for d-3-hydroxybutyrate dehydrogenase (HBDH) was cloned from Pseudomonas fragi. The nucleotide sequence contained a 780 bp open reading frame encoding a 260 amino acid residue protein. The recombinant enzyme was efficiently expressed in Escherichia coli cells harboring pHBDH11 and was purified to homogeneity as judged by SDS-PAGE. The enzyme showed a strict stereospecificity to the D-enantiomer (3R-configuration) of 3-hydroxybutyrate as a substrate. Crystals of the ligand-free HBDH and of the enzyme-NAD+ complex were obtained using the hanging-drop, vapor-diffusion method. The crystal structure of the HBDH was solved by the multiwavelength anomalous diffraction method using the SeMet-substituted enzyme and was refined to 2.0 A resolution. The overall structure of P.fragi HBDH, including the catalytic tetrad of Asn114, Ser142, Tyr155, and Lys159, shows obvious relationships with other members of the short-chain dehydrogenase/reductase (SDR) family. A cacodylate anion was observed in both the ligand-free enzyme and the enzyme-NAD+ complex, and was located near the catalytic tetrad. It was shown that the cacodylate inhibited the NAD+-dependent D-3-hydroxybutyrate dehydrogenation competitively, with a Ki value of 5.6 mM. From the interactions between cacodylate and the enzyme, it is predicted that substrate specificity is achieved through the recognition of the 3-methyl and carboxyl groups of the substrate.

PubMed: 16325199
DOI: 10.1016/j.jmb.2005.10.072

実験手法

X-RAY DIFFRACTION (2 Å)