1WJF

SOLUTION STRUCTURE OF H12C MUTANT OF THE N-TERMINAL ZN BINDING DOMAIN OF HIV-1 INTEGRASE COMPLEXED TO CADMIUM, NMR, 40 STRUCTURES

1WJF の概要

• エントリーDOI: 10.2210/pdb1wjf/pdb
• 分子名称: HIV-1 INTEGRASE, CADMIUM ION (2 entities in total)
• 機能のキーワード: zn-binding protein, aids, polyprotein, hydrolase, aspartyl protease
• 由来する生物種: Human immunodeficiency virus 1
• 細胞内の位置: Gag-Pol polyprotein: Host cell membrane; Lipid-anchor. Matrix protein p17: Virion membrane; Lipid- anchor . Capsid protein p24: Virion . Nucleocapsid protein p7: Virion . Reverse transcriptase/ribonuclease H: Virion . Integrase: Virion : P04587
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12548.72

構造登録者

Cai, M.,Gronenborn, A.M.,Clore, G.M. (登録日: 1998-06-11, 公開日: 1998-12-16, 最終更新日: 2024-05-01)

主引用文献

Cai, M.,Huang, Y.,Caffrey, M.,Zheng, R.,Craigie, R.,Clore, G.M.,Gronenborn, A.M.
Solution structure of the His12 --> Cys mutant of the N-terminal zinc binding domain of HIV-1 integrase complexed to cadmium.
Protein Sci., 7:2669-2674, 1998

PubMed Abstract: The solution structure of His12 --> Cys mutant of the N-terminal zinc binding domain (residues 1-55; IN(1-55)) of HIV-1 integrase complexed to cadmium has been solved by multidimensional heteronuclear NMR spectroscopy. The overall structure is very similar to that of the wild-type N-terminal domain complexed to zinc. In contrast to the wild-type domain, however, which exists in two interconverting conformational states arising from different modes of coordination of the two histidine side chains to the metal, the cadmium complex of the His12 --> Cys mutant exists in only a single form at low pH. The conformation of the polypeptide chain encompassing residues 10-18 is intermediate between the two forms of the wild-type complex.

PubMed: 9865962

実験手法

SOLUTION NMR