1WEK

Crystal structure of the conserved hypothetical protein TT1465 from Thermus thermophilus HB8

Summary for 1WEK

• Entry DOI: 10.2210/pdb1wek/pdb
• Descriptor: hypothetical protein TT1465, PHOSPHATE ION (3 entities in total)
• Functional Keywords: rossmann fold, structural genomics, riken structural genomics/proteomics initiative, rsgi, unknown function
• Biological source: Thermus thermophilus
• Total number of polymer chains: 6
• Total formula weight: 147555.24

Authors

Kukimoto-Niino, M.,Murayama, K.,Kato-Murayama, M.,Terada, T.,Shirouzu, M.,Kuramitsu, S.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2004-05-25, release date: 2004-11-25, Last modification date: 2014-11-12)

Primary citation

Kukimoto-Niino, M.,Murayama, K.,Kato-Murayama, M.,Idaka, M.,Bessho, Y.,Tatsuguchi, A.,Ushikoshi-Nakayama, R.,Terada, T.,Kuramitsu, S.,Shirouzu, M.,Yokoyama, S.
Crystal structures of possible lysine decarboxylases from Thermus thermophilus HB8
Protein Sci., 13:3038-3042, 2004

PubMed Abstract: TT1887 and TT1465 from Thermus thermophilus HB8 are conserved hypothetical proteins, and are annotated as possible lysine decarboxylases in the Pfam database. Here we report the crystal structures of TT1887 and TT1465 at 1.8 A and 2.2 A resolutions, respectively, as determined by the multiwavelength anomalous dispersion (MAD) method. TT1887 is a homotetramer, while TT1465 is a homohexamer in the crystal and in solution. The structures of the TT1887 and TT1465 monomers contain single domains with the Rossmann fold, comprising six alpha helices and seven beta strands, and are quite similar to each other. The major structural differences exist in the N terminus of TT1465, where there are two additional alpha helices. A comparison of the structures revealed the elements that are responsible for the different oligomerization modes. The distributions of the electrostatic potential on the solvent-accessible surfaces suggested putative active sites.

PubMed: 15459330
DOI: 10.1110/ps.041012404

Experimental method

X-RAY DIFFRACTION (2.2 Å)